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Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Mouse anti-Human BMP8b Monoclonal Antibody | anti-BMP8b antibody

BMP8b antibody

Gene Names
BMP8B; OP2; BMP8
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
BMP8b; Monoclonal Antibody; BMP8b antibody; Bone morphogenetic protein 8B; BMP8; OP2; anti-BMP8b antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT13E6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
402
Applicable Applications for anti-BMP8b antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human BMP8b (264-402aa) purified from E. coli
Clone Note
Anti-human BMP8b mAb, clone AT13E6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BMP8b protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Immunofluorescence (IF)

(ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with ATGA0332 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Immunofluorescence (IF) (ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with ATGA0332 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Related Product Information for anti-BMP8b antibody
Bone morphogenic proteins (BMPs) are members of the TGF beta superfamily. BMPs are involved in the induction of cartilage and bone formation. Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. BMP-8 is thought to be involved in early development, as detectable expression has not been found in adult organs. Two BMP-8 proteins exist, namely BMP-8A and BMP-8B (also designated OP-2), and are encoded by two distinct genes.
Product Categories/Family for anti-BMP8b antibody
References
Ozkaynak E., et al. (1992) J Biol Chem. 267 (35):25220-25227; Gregory SG., et al. (2006) Nature. 441 (7091):315-321.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
656
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,151 Da
NCBI Official Full Name
bone morphogenetic protein 8B preproprotein
NCBI Official Synonym Full Names
bone morphogenetic protein 8b
NCBI Official Symbol
BMP8B
NCBI Official Synonym Symbols
OP2; BMP8
NCBI Protein Information
bone morphogenetic protein 8B
UniProt Protein Name
Bone morphogenetic protein 8B
UniProt Gene Name
BMP8B
UniProt Synonym Gene Names
BMP8; BMP-8; BMP-8B; OP-2

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The encoded protein stimulates thermogenesis in brown adipose tissue. Expression of this gene may be downregulated in pancreatic cancer. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 1. [provided by RefSeq, Jul 2016]

Uniprot Description

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis ().

Research Articles on BMP8b

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Product Notes

The BMP8b bmp8b (Catalog #AAA200288) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BMP8b antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BMP8b can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the BMP8b bmp8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMP8b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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