Mouse anti-Human BMP-4 Monoclonal Antibody | anti-BMP-4 antibody

Mouse Anti-Human BMP-4

Cat. Num. AAA690973

• Gene Names: BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• Reactivity: Human
• Purity: Protein G chromatography
• Synonyms: BMP-4; Monoclonal Antibody; Mouse Anti-Human BMP-4; Anti-human BMP-4; anti-BMP-4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2
• Purity/Purification: Protein G chromatography
• Form/Format: Lyophilized
• Sequence Length: 408

• Application Notes: Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.
• Antibody Generation: Produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen. This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
• Buffer: PBS, pH 7.2
• Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (5.0 ng/ml), a concentration of 0.4-0.6 ug/ml of this antibody is required.

Related Product Information for anti-BMP-4 antibody

BMP-4 is a TGFbeta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein. Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP-4/BMP-7 disulfide-linked heterodimer. Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/ BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation. In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm. BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.

NCBI and Uniprot Product Information

• NCBI GI #: 157276593
• NCBI GeneID: 652
• NCBI Accession #: NP_001193.2
• NCBI GenBank Nucleotide #: NM_001202.3
• UniProt Accession #: P12644; Q9UM80
• Molecular Weight: 46,555 Da
• NCBI Official Full Name: bone morphogenetic protein 4 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: BMP4
• NCBI Official Synonym Symbols: ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• NCBI Protein Information: bone morphogenetic protein 4; BMP-4; BMP-2B; bone morphogenetic protein 2B
• UniProt Protein Name: Bone morphogenetic protein 4
• UniProt Gene Name: BMP4
• UniProt Synonym Gene Names: BMP2B; DVR4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction

By similarity.

Subunit structure: Homodimer; disulfide-linked

By similarity. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition

By similarity. Interacts with SOSTDC1. Ref.5

Subcellular location: Secreted › extracellular space › extracellular matrix.

Tissue specificity: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

Involvement in disease: Microphthalmia, syndromic, 6 (MCOPS6) [MIM:607932]: A disease characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies. Microphthalmia is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7Non-syndromic orofacial cleft 11 (OFC11) [MIM:600625]: A birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9

Sequence similarities: Belongs to the TGF-beta family.