Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group B Monoclonal Antibody (HEB-20).)
Mouse Blood Group Antigen B Monoclonal Antibody | anti-ABO antibody
Blood Group Antigen B (CD173) Mouse Monoclonal Antibody
Gene Names
ABO; GTB; NAGAT; A3GALNT; A3GALT1
Reactivity
Human. others-not known
Applications
Immunofluorescence, Immunohistochemistry
Synonyms
Blood Group Antigen B; Monoclonal Antibody; Blood Group Antigen B (CD173) Mouse Monoclonal Antibody; A transferase; ABO; B transferase; CD173; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Histo-blood group A transferase; Histo-blood group B transferase; NAGAT; anti-ABO antibody
Host
Mouse
Reactivity
Human. others-not known
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
HEB-20
Specificity
The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood. The MAb HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This MAb is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
354
Applicable Applications for anti-ABO antibody
Agglutination; Immunofluorescence; Immunohistology (Formalin)
Application Notes
Agglutination
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Cellular Localization
Cell Surface
Immunogen
Mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B
Mol. Weight of Antigen
Multiple
Hu-Chromosome Location
9q34.2
Positive Control
KG1 cells or human colorectal carcinoma tissues.
Supplied As
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0ml
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group B Monoclonal Antibody (HEB-20).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group B Monoclonal Antibody (HEB-20).)
References
Van-k J, Dr-malov- D, Smyslov- O, Nmec M, Viklick- V, Wisniewski K. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma. 1989;36(4):479-88
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
histo-blood group ABO system transferase
NCBI Official Synonym Full Names
ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)
NCBI Official Symbol
ABO
NCBI Official Synonym Symbols
GTB; NAGAT; A3GALNT; A3GALT1
NCBI Protein Information
histo-blood group ABO system transferase
UniProt Protein Name
Histo-blood group ABO system transferase
Protein Family
UniProt Gene Name
ABO
UniProt Synonym Gene Names
A transferase; B transferase
UniProt Entry Name
BGAT_HUMAN
NCBI Description
This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ABO: This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Belongs to the glycosyltransferase 6 family.
Protein type: EC 2.4.1.40; Transferase; EC 2.4.1.37; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series
Chromosomal Location of Human Ortholog: 9q34.2
Disease: Blood Group, Abo System
Research Articles on ABO
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Product Notes
The ABO abo (Catalog #AAA439591) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Blood Group Antigen B (CD173) Mouse Monoclonal Antibody reacts with Human. others-not known and may cross-react with other species as described in the data sheet. AAA Biotech's Blood Group Antigen B can be used in a range of immunoassay formats including, but not limited to, Agglutination; Immunofluorescence; Immunohistology (Formalin). Agglutination Immunofluorescence (0.5-1ug/ml) Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the ABO abo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Blood Group Antigen B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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