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Western Blot (WB)
(Western Blot: Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using MBS600028: murine pre-B cell lysate (negative control).)
Mouse anti-Human, Mouse Blimp-1 Monoclonal Antibody | anti-Blimp-1 antibody
Blimp-1 (BLIMP 1, BLIMP1, B Lymphocyte Induced Maturation Protein 1, Positive Regulatory Domain I Binding Factor 1, PR Domain Containing 1, PRDI BF1, PRDM1)
Gene Names
Blimp-1; blimp-1; CG5249; dBlimp-1; dBlimp1; DmelCG5249
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Blimp-1; Monoclonal Antibody; Blimp-1 (BLIMP 1; BLIMP1; B Lymphocyte Induced Maturation Protein 1; Positive Regulatory Domain I Binding Factor 1; PR Domain Containing 1; PRDI BF1; PRDM1); Anti -Blimp-1 (BLIMP 1; anti-Blimp-1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8J333
Specificity
Recognizes mouse Blimp-1 at ~98kD and ~80kD (beta form). Species Crossreactivity: human and porcine (see Specific Reference).
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 150mM sodium chloride, 0.1% sodium azide.
Applicable Applications for anti-Blimp-1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Dilution: Immunofluorescence/Immunocytochemistry: 1:50-1:200
Flow Cytometry: 8ug/ml where cells are used at 2x10e6/ml
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:200
Dilution: Immunofluorescence/Immunocytochemistry: 1:50-1:200
Flow Cytometry: 8ug/ml where cells are used at 2x10e6/ml
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:200
Immunogen
A fragment of the mouse Blimp-1 (residues 199-409).
Cellular Localization
Nuclear
Positive Control
P3X (murine plasmocytoma) cell lysates
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot: Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using MBS600028: murine pre-B cell lysate (negative control).)
Western Blot (WB)
(Western Blot: Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using MBS600028: murine pre-B cell lysate (negative control).)
Flow Cytometry (FC/FACS)
(Flow cytometry / FACS analysis: using MBS600028 - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells.)
Flow Cytometry (FC/FACS)
(Flow cytometry / FACS analysis: using MBS600028 - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells.)
Flow Cytometry (FC/FACS)
(Flow cytometry / FACS analysis: using [B2106-70] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice.)
Flow Cytometry (FC/FACS)
(Flow cytometry / FACS analysis: using [B2106-70] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice.)
Flow Cytometry (FC/FACS)
(Flow cytometry / FACS analysis: Blimp-1 using B2106-70] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line).)
Flow Cytometry (FC/FACS)
(Flow cytometry / FACS analysis: Blimp-1 using B2106-70] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line).)
Immunofluorescence (IF)
(Immunoflourescence: using MBS600028 Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10µm). Positive surface epithelium and centrocytes are labelled.)
Immunofluorescence (IF)
(Immunoflourescence: using MBS600028 Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10µm). Positive surface epithelium and centrocytes are labelled.)
Related Product Information for anti-Blimp-1 antibody
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
Product Categories/Family for anti-Blimp-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
133,280 Da
NCBI Official Full Name
Blimp-1
NCBI Official Symbol
Blimp-1
NCBI Official Synonym Symbols
blimp-1; CG5249; dBlimp-1; dBlimp1; DmelCG5249
NCBI Protein Information
CG5249 gene product from transcript CG5249-RA; Blimp-1; CG5249-PA; Blimp-1-PA; dBlimp1
UniProt Protein Name
Blimp-1
UniProt Gene Name
Blimp-1
UniProt Entry Name
Q9VRN4_DROME
Uniprot Description
Sequence similarities: Contains 1 SET domain. RuleBase RU004538
Research Articles on Blimp-1
Similar Products
Product Notes
The Blimp-1 blimp-1 (Catalog #AAA600028) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Blimp-1 (BLIMP 1, BLIMP1, B Lymphocyte Induced Maturation Protein 1, Positive Regulatory Domain I Binding Factor 1, PR Domain Containing 1, PRDI BF1, PRDM1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Blimp-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Dilution: Immunofluorescence/Immunocytochemistry: 1:50-1:200 Flow Cytometry: 8ug/ml where cells are used at 2x10e6/ml Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:200. Researchers should empirically determine the suitability of the Blimp-1 blimp-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Blimp-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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