Rat anti-Mouse BiP Monoclonal Antibody | anti-HSPA5 antibody

Rat Anti-Mouse BiP-UNLB

Cat. Num. AAA673495

• Gene Names: HSPA5; BIP; MIF2; GRP78; FLJ26106
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Synonyms: BiP; Monoclonal Antibody; Rat Anti-Mouse BiP-UNLB; Rat Anti-Mouse BiP - Purified; Unlabeled Antibody; Rat Anti-Mouse BiP; anti-HSPA5 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1 kappa
• Clone Number: 76-E6
• Specificity: BiP (Immunoglobulin heavy chain binding protein, Mr 78 kDa); The immunoglobulin heavy chain binding protein BiP (Binding Protein, [1]) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78.2 While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as "molecular chaperones," which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding.1-5 With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (u+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP.7 Monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP.8
• Form/Format: Purified (UNLB) Antibody
• Sequence Length: 653

• Applicable Applications for anti-HSPA5 antibody: ELISA; Western Blot
• Application Notes: Research Application(s): Studies of immunoglobulin assembly and secretion
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform with characteristics of a standard reference reagent using Western blotting. Representative data are included in this data sheet.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Bole, D.G., L.M. Hendershot, and J.F. Kearney. 1986. J. Cell Biol. 102:1558.
2. Haas, I.G. 1991. Current Topics Micro. & Immunol. 167:71.
3. Hendrick, J.P. and Hartl, F.U. 1991. FASEB J. 9(15):1559.
4. Pierce, S.K. 1994. Experientia 50(11-12):1026
5. Haas, I.G. 1994. Experientia 50(11-12):1012.
6. Wu, Q. 1996.
7. Kassenbrock, C.K. and Kelly, R.B. 1989. EMBO J. 8: 233.

NCBI and Uniprot Product Information

• NCBI GI #: 1143492
• NCBI GeneID: 3309
• UniProt Accession #: P11021; Q2EF78; Q9NPF1; Q9UK02; B0QZ61
• Molecular Weight: 72,333 Da
• NCBI Official Full Name: BiP
• NCBI Official Synonym Full Names: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78; FLJ26106
• NCBI Protein Information: 78 kDa glucose-regulated protein; OTTHUMP00000022124; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
• UniProt Protein Name: 78 kDa glucose-regulated protein
• Protein Family: Biphenyl dioxygenase
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78
• UniProt Entry Name: GRP78_HUMAN

NCBI Description

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.

Uniprot Description

Function: Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Ref.12

Subunit structure: Interacts with DNAJC1 (via J domain)

By similarity. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A and TRIM21. May form a complex with ERLEC1, OS9, SEL1L and SYVN1. Ref.16 Ref.20 Ref.21

Subcellular location: Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.17 Ref.18

Involvement in disease: Note=Autoantigen in rheumatoid arthritis. Ref.10

Sequence similarities: Belongs to the heat shock protein 70 family.