Mouse anti-Human Bid Monoclonal Antibody | anti-BID antibody
Mouse Anti-Human Bid-UNLB
Members of the Bcl-2 family are pro- or antiapoptotic proteins that process one or several of the Bcl-2 homology (BH) domains. Bid is a proapoptotic, BH3-domain-only member or BH3-only protein (BOP) of the Bcl-2 family. Bid is localized in the cytosolic fraction of living cells as an inactive precursor. Activation of Bid occurs upon its cleavage by caspase-8 in the Fas and TNF- alpha apoptotic signaling pathways. Recent studies have suggested that Bid is also cleaved and activated by granzyme B, calpain and lysosomal extracts. Cleaved Bid translocates to mitochondria where it induces the release of cytochrome c from the intermembrane space of the mitochondria. The release of cytochrome c initiates apoptosis by activating downstream caspases through binding to Apaf-1. Bid has also been shown to interact with with Bax, another Bcl-2 family protein, to trigger a conformational change in Bax leading to the dimerization and integration of Bax into the outer mitochondrial membrane and activating the release of cytochrome c.1-10
Reagents are stable for the period shown on the label if stored as directed.
2. Wang, K., X.M. Yin, D.T. Chao, C.L. Milliman, and S.J. Korsmeyer. 1996. Genes Dev. 10:2859.
3. Li, H., H. Zhu, C. Xu, and J. Yuan. 1998. Cell 94:491.
4. Kudla, G., S. Montessuit, R. Eskes, C. Berrier, J. Martinou, A. Ghazi, and B. Antonsson. 2000. J. Biol. Chem. 275:22713.
5. Gross, A., X. Yin, K. Wang, M.C. Wei, J. Jockel, C. Milliman, H. Erdjument, P. Tempst, and S.J. Korsmeyer. 1999. J. Biol. Chem. 274:1156.
6. Chen, M., H. He, S. Zhan, S. Krajewski, J.C. Reed, and R.A. Gottlieb. 2001. J. Biol. Chem. 276:30724.
7. Cirman, T., K. Oresic, G.D. Mazovec, V. Turk, J.C. Reed, R.M. Myers, G.S. Salvesen, and B. Turk. 2004. J. Biol. Chem. 279:3578.
8. Stoka, V., B. Turk, S.L. Schendel, T. Kim, T. Cirman, S.J. Snipas, L.M. Ellerby, D. Bredesen, H. Freeze, M. Abrahamson, D. Bromme, S. Krajewski, J.C. Reed, X. Yin, V. Turk, and G.S. Salvesen. 2001. J. Biol. Chem. 276:3149.
9. Eskes, R., S. Desagher, B. Antonsson, and J. Martinou. 2000. Mol. Cell. Biol. 20:929.
10. Barry, M., J.A. Heibein, M.J. Pinkiski, S. Lee, R.W. Moyer, D.R. Green, and R.C. Bleackley. 2000. Mol. Cell. Biol. 20:3781.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq]
Uniprot Description
Function: The major proteolytic product p15 BID allows the release of cytochrome c
By similarity. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. Ref.3
Subunit structure: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2
By similarity. p15 BID interacts with ITCH. Ref.11
Subcellular location: Cytoplasm
By similarity. Mitochondrion membrane
By similarity. Note: When uncleaved, it is predominantly cytoplasmic. Ref.3BH3-interacting domain death agonist p15: Mitochondrion membrane
By similarity. Note: Translocates to mitochondria as an integral membrane protein
By similarity. Ref.3BH3-interacting domain death agonist p13: Mitochondrion membrane
By similarity. Note: Associated with the mitochondrial membrane
By similarity. Ref.3Isoform 1: Cytoplasm Ref.3. Isoform 3: Cytoplasm Ref.3. Isoform 2: Mitochondrion membrane. Note: A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. Ref.3
Tissue specificity: Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). Ref.3
Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Post-translational modification: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products
By similarity.Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.9 Ref.10p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
Sequence caution: The sequence AAH22072.2 differs from that shown. Reason: Erroneous initiation.
Research Articles on BID
Similar Products
Product Notes
The BID bid (Catalog #AAA670134) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human Bid-UNLB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bid can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoprecipitation; Western Blot. Researchers should empirically determine the suitability of the BID bid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bid, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
