Mouse BHMT Monoclonal Antibody | anti-BHMT antibody

BHMT antibody

Cat. Num. AAA830433

• Gene Names: BHMT; BHMT1; HEL-S-61p
• Applications: Immunohistochemistry, Western Blot
• Purity: BHMT antibody was purified by affinity chromatography.
• Synonyms: BHMT; Monoclonal Antibody; BHMT antibody; Monoclonal BHMT; Anti-BHMT; BHMT (betaine-homocysteine methyltransferase); EC 2.1.1.5; betaine--homocysteine S-methyltransferase 1; betaine-homocysteine methyltransferase betaine homocysteine methyltransferase; betaine homocysteine S methyltransferase; betaine homocysteine S methyltransferase 1; BHMT1; anti-BHMT antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Clone Number: 2F6
• Purity/Purification: BHMT antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 406

• Applicable Applications for anti-BHMT antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50; WB: 1:1000
• Biological Significance: Betaine homocysteine methyltransferase (BHMT), is a cytosolic enzyme, its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.
• Cross-Reactivity: Human
• Immunogen: BHMT antibody was raised in mouse using a full length recombinant protein of human BHMT (NP_001704) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BHMT protein (Right) or empty vector (Left) detected with BHMT antibody)

Related Product Information for anti-BHMT antibody

Mouse monoclonal BHMT antibody

Product Categories/Family for anti-BHMT antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 157266337
• NCBI GeneID: 635
• NCBI Accession #: NP_001704
• NCBI GenBank Nucleotide #: NM_001713.2
• UniProt Accession #: Q93088; Q9UNI9
• Molecular Weight: 44,998 Da
• NCBI Official Full Name: betaine--homocysteine S-methyltransferase 1
• NCBI Official Synonym Full Names: betaine--homocysteine S-methyltransferase
• NCBI Official Symbol: BHMT
• NCBI Official Synonym Symbols: BHMT1; HEL-S-61p
• NCBI Protein Information: betaine--homocysteine S-methyltransferase 1
• UniProt Protein Name: Betaine--homocysteine S-methyltransferase 1
• Protein Family: Betaine--homocysteine S-methyltransferase
• UniProt Gene Name: BHMT
• UniProt Entry Name: BHMT1_HUMAN

NCBI Description

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: Methyltransferase; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - glycine, serine and threonine; EC 2.1.1.5

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding

Biological Process: S-methylmethionine cycle; protein amino acid methylation; betaine catabolic process; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; betaine metabolic process; regulation of homocysteine metabolic process