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Mouse Betaine Homocysteine S-Methyltransferase Monoclonal Antibody | anti-BHMT antibody

Mouse Anti Human Betaine Homocysteine S-Methyltransferase

Gene Names
BHMT; BHMT1; HEL-S-61p
Applications
ELISA, Western Blot
Purity
BHMT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Synonyms
Betaine Homocysteine S-Methyltransferase; Monoclonal Antibody; Mouse Anti Human Betaine Homocysteine S-Methyltransferase; BHMT Antibody; Mouse Anti Human; BHMT; Betaine Homocysteine S-Methyltransferase 1; BHMT1; anti-BHMT antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a heavy chain and kappa light chain
Clone Number
P3D6AT
Purity/Purification
BHMT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sterile filtered colorless solution.
Sequence Length
406
Applicable Applications for anti-BHMT antibody
ELISA (EIA), Western Blot (WB)
Application Notes
BHMT antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Immunogen
Anti-human BHMT mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human BHMT amino acids 1-406 purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
Related Product Information for anti-BHMT antibody
Introduction: Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT displays differential expression in a model of liver cirrhosis.
Product Categories/Family for anti-BHMT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
635
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,998 Da
NCBI Official Full Name
betaine--homocysteine S-methyltransferase 1
NCBI Official Synonym Full Names
betaine--homocysteine S-methyltransferase
NCBI Official Symbol
BHMT
NCBI Official Synonym Symbols
BHMT1; HEL-S-61p
NCBI Protein Information
betaine--homocysteine S-methyltransferase 1; epididymis secretory sperm binding protein Li 61p
UniProt Protein Name
Betaine--homocysteine S-methyltransferase 1
UniProt Gene Name
BHMT
UniProt Entry Name
BHMT1_HUMAN

NCBI Description

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: EC 2.1.1.5; Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding

Biological Process: S-methylmethionine cycle; protein amino acid methylation; betaine catabolic process; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; betaine metabolic process; regulation of homocysteine metabolic process

Research Articles on BHMT

Similar Products

Product Notes

The BHMT bhmt (Catalog #AAA140215) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Betaine Homocysteine S-Methyltransferase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). BHMT antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the BHMT bhmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Betaine Homocysteine S-Methyltransferase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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