Mouse Beta II tubulin Monoclonal Antibody | anti-TUBB2A antibody

Beta II tubulin mouse monoclonal antibody

Cat. Num. AAA9611249

• Gene Names: TUBB2A; TUBB; TUBB2; CDCBM5
• Reactivity: Human; Mouse; Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity-chromatography.
• Synonyms: Beta II tubulin; Monoclonal Antibody; Beta II tubulin mouse monoclonal antibody; Beta II tubulin antibody; β II Tubulin;Beta2;TBB4B_HUMAN;TUBB 2;TUBB2 ;Tubb4b;Tubulin beta 2;; anti-TUBB2A antibody

• Host: Mouse
• Reactivity: Human; Mouse; Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Beta II tubulin antibody detects endogenous levels of Beta II tubulin.
• Purity/Purification: Affinity-chromatography.
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 445

• Applicable Applications for anti-TUBB2A antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB(1:1000-1:10000), IP(1:200)
• Immunogen: Purified recombinant fragment of human Beta II tubulin expressed in E. Coli.
• Fragment: Fab fragment
• Conjugation: Unconjugated
• Storage Buffer: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C. Stable for 12 months from date of receipt.
• Post Translational Modifications: Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
• Preparation and Storage: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-TUBB2A antibody

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

Similarity: Belongs to the tubulin family.

NCBI and Uniprot Product Information

• NCBI GI #: 49456871
• NCBI GeneID: 7280
• UniProt Accession #: Q13885
• Molecular Weight: Observed Mol.Wt.: 55kDa. Predicted Mol.Wt.: 50kDa.
• NCBI Official Full Name: TUBB, partial
• NCBI Official Synonym Full Names: tubulin beta 2A class IIa
• NCBI Official Symbol: TUBB2A
• NCBI Official Synonym Symbols: TUBB; TUBB2; CDCBM5
• NCBI Protein Information: tubulin beta-2A chain
• UniProt Protein Name: Tubulin beta-2A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB2A
• UniProt Synonym Gene Names: TUBB2
• UniProt Entry Name: TBB2A_HUMAN

NCBI Description

Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. The protein encoded by this gene is a beta-tubulin. Defects in this gene are associated with complex cortical dysplasia with other brain malformations-5. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

Uniprot Description

TUBB2A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: microtubule; cytoplasm; nucleus; vesicle

Molecular Function: GTPase activity; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 5