Testing Data
(All lanes : Anti-ACTB Antibody at 1:1000 dilutionnLane 1: Hela whole cell lysatenLane 2: HepG2 whole cell lysatenLane 3: NIH-3T3 whole cell lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 42 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse beta-Actin Monoclonal Antibody | anti-ACTB antibody
Anti-beta-Actin Mouse mAb
ICC: 1:10-50
IHC: 1:25
FC/FACS: 1:25
Testing Data
(All lanes : Anti-ACTB Antibody at 1:1000 dilutionnLane 1: Hela whole cell lysatenLane 2: HepG2 whole cell lysatenLane 3: NIH-3T3 whole cell lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 42 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes : Anti-ACTB Antibody at 1:1000 dilutionnLane 1: Hela whole cell lysatenLane 2: HepG2 whole cell lysatenLane 3: NIH-3T3 whole cell lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 42 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ACTB Antibody with Hela cell followed by Alexa Fluor? 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ACTB Antibody with Hela cell followed by Alexa Fluor? 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
Testing Data
(AM1829b staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval wa)
Testing Data
(AM1829b staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval wa)
Testing Data
(Overlay histogram showing A431 cells stained with AM1829b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-speci)
Testing Data
(Overlay histogram showing A431 cells stained with AM1829b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-speci)
Testing Data
(All lanes : Anti-ACTB Antibody at 1:1000 dilutionnLane 1: A431 whole cell lysatenLane 2: C2C12 whole cell lysatenLane 3: C6 whole cell lysatenLane 4: Hela whole cell lysatenLane 5: MCF-7 whole cell lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 42 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes : Anti-ACTB Antibody at 1:1000 dilutionnLane 1: A431 whole cell lysatenLane 2: C2C12 whole cell lysatenLane 3: C6 whole cell lysatenLane 4: Hela whole cell lysatenLane 5: MCF-7 whole cell lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size : 42 kDannBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody . AM1829b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody . AM1829b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
Uniprot Description
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.MiscellaneousIn vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.CautionWas originally thought to be part of the MLL5-L complex, at least composed of KMT2E, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT (PubMed:19377461). However, the corresponding article has been retracted (PubMed:24336203).
Research Articles on ACTB
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Product Notes
The ACTB actb (Catalog #AAA477336) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-beta-Actin Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Actin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF). WB: 1:1000 ICC: 1:10-50 IHC: 1:25 FC/FACS: 1:25. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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