Western Blot (WB)
(Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2. Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.)
Mouse beta 4 Spectrin Monoclonal Antibody | anti-SPTBN4 antibody
Mouse Anti-Human beta 4 Spectrin Monoclonal IgG1
Western Blot (WB)
(Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2. Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.)
Western Blot (WB)
(Western Blot analysis of COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2. Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.)
NCBI and Uniprot Product Information
NCBI Description
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SPTBN4: Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19q13.13
Cellular Component: adherens junction; axon hillock; cell soma; cytoplasm; cytosol; membrane; nuclear matrix; paranode region of axon; plasma membrane; PML body; spectrin
Molecular Function: actin binding; ankyrin binding; phosphatase binding; phospholipid binding; protein binding; spectrin binding; structural constituent of cytoskeleton
Biological Process: actin filament capping; activation of MAPKK activity; adult walking behavior; axon guidance; axonogenesis; cellular protein metabolic process; central nervous system projection neuron axonogenesis; clustering of voltage-gated sodium channels; cytoskeletal anchoring; epidermal growth factor receptor signaling pathway; ER to Golgi vesicle-mediated transport; fertilization; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; negative regulation of heart rate; nerve growth factor receptor signaling pathway; positive regulation of multicellular organism growth; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; Ras protein signal transduction; regulation of peptidyl-serine phosphorylation; regulation of sodium ion transport; sensory perception of sound; small GTPase mediated signal transduction; transmission of nerve impulse; vascular endothelial growth factor receptor signaling pathway; vesicle-mediated transport
Research Articles on SPTBN4
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Product Notes
The SPTBN4 sptbn4 (Catalog #AAA806977) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human beta 4 Spectrin Monoclonal IgG1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's beta 4 Spectrin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). Western Blot: 1:1000. Researchers should empirically determine the suitability of the SPTBN4 sptbn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta 4 Spectrin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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