Mouse Beta-3 integrin subunit Monoclonal Antibody | anti-Itgb3 antibody

Mouse Anti-Human Beta-3 integrin subunit Monoclonal Antibody

Cat. Num. AAA584087

• Gene Names: Itgb3; CD61; GP3A; INGRB3
• Reactivity: Reactivity: Human. Cross reactivity: Bovine
• Applications: Immunoprecipitation, Western Blot
• Synonyms: Beta-3 integrin subunit; Monoclonal Antibody; Mouse Anti-Human Beta-3 integrin subunit Monoclonal Antibody; Platelet membrane glycoprotein IIIa; GPIIIa; anti-Itgb3 antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Bovine
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 25

• Applicable Applications for anti-Itgb3 antibody: F, FS, Immunoassay (IA), Immunoprecipitation (IP), P, Western Blot (WB)
• Application Notes: Application Notes: IHC-F:- Tissue sections were fixed in acetone. As negative control an irrelevant mouse IgG was used (Ref.2).; Application Use: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in users experimental setting.
• Positive Control: Endometrium
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-Itgb3 antibody

Product Description: The monoclonal antibody BV4 recognizes human beta3 integrin subunit present in Platelet glycoprotein GPIIb-IIIa (integrin alphaIIb/beta3, CD41/CD61) and in the vitronectin receptor (integrin alphaV/beta3, CD51/CD61). Intergins are a family of heterodimeric membrane glycoproteins expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. As adhesion molecules they play an important role in numerous biological processes such as platelet aggregation, inflammation, immune function, wound healing, tumour metastasis and tissue migration during embryogenesis. In addition integrins are involved in signaling pathways, transmitting signals both into an out from cells. All integrins consist of two non-covalently associated subunits, alpha and beta. At least 12 different alpha subunits and 8 beta subunits have been identified. The beta subunits all contain 56 conserved cysteines (except beta4 which has 48) which are arranged in four repeating units. The beta3 subunit is a 93kDa protein that contains a large loop in the N-terminus stabilized by intrachain disulphide bonding with the first cysteine-rich repeat.
Platelet glycoprotein GPIIb-IIIa is expressed on platelets and megakaryoblasts. It is constitutively expressed and becomes activated on triggered platelets. Platelet glycoprotein GPIIb-IIIa binds to fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. Next to this it is also a receptor for several soluble adhesive proteins.Vitronectin receptor is expressed on endothelial cells, some B cells, monocytes/macrophages, platelets and tumour cells. Vitronectin receptor binds next to vitronectin to fibrinogen, vWF, thrombospondin, fibronectin, osteopontin and collagen. Defects in human beta3 integrin are a cause of Glanzmann thrombasthenia, which is an autosomal recessive disorder characterized by mucocutaneous bleeding and the inability of this integrin to recognize macromolecular or synthetic peptide ligands.

References

1. Soldi, R et al; Role of alpha v beta 3 integrin in the activation of vascular endothelial growth factor receptor-2. EMBO J 1999, 18:882 2. Kimmins, S eta l: Immunohistochemical localization of integrin alpha v beta 3 and osteopontin suggests that they do not interact during embryo implantation in ruminants. Reprod Biol and Endocrinol. 2004, 2:19
3. Neto, D et al; Alpha-v-beta3 integrin expression in melanocytic nevi and cutaneous melanoma. J Cutan Pathol. 2007, 34: 851
4. Tang, N et al; N-terminal and C-terminal heparin-binding domain polypeptides derived from fibronectin reduce adhesion and invasion of liver cancer cells. BMC Cancer 2010, 10: 552

NCBI and Uniprot Product Information

• NCBI GI #: 2736196
• NCBI GeneID: 16416
• Molecular Weight: 86,738 Da
• NCBI Official Full Name: beta3 integrin, partial
• NCBI Official Synonym Full Names: integrin beta 3
• NCBI Official Symbol: Itgb3
• NCBI Official Synonym Symbols: CD61; GP3A; INGRB3
• NCBI Protein Information: integrin beta-3; GPIIIa; platelet gpIIIa; platelet membrane glycoprotein IIIa
• UniProt Protein Name: Integrin beta-3
• Protein Family: Integrin
• UniProt Gene Name: Itgb3
• UniProt Synonym Gene Names: GPIIIa
• UniProt Entry Name: ITB3_MOUSE

Uniprot Description

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: filopodium membrane; focal adhesion; cell surface; integral to membrane; cell projection; membrane; microvillus membrane; apical plasma membrane; melanosome; plasma membrane; integrin complex; nucleus; cell junction; receptor complex; external side of plasma membrane

Molecular Function: integrin binding; identical protein binding; protein binding; protease binding; extracellular matrix binding; vascular endothelial growth factor receptor 2 binding; fibronectin binding; protein disulfide isomerase activity; receptor activity; cell adhesion molecule binding; peptide binding

Biological Process: negative regulation of lipid transport; entry of virus into host cell; protein folding; positive regulation of osteoclast differentiation; positive regulation of leukocyte migration; positive regulation of smooth muscle cell proliferation; cell-matrix adhesion; multicellular organismal development; smooth muscle cell migration; negative chemotaxis; positive regulation of smooth muscle cell migration; regulation of cell migration; positive regulation of MAPKKK cascade; cell-substrate junction assembly; positive regulation of bone resorption; cell adhesion; cell growth; integrin-mediated signaling pathway; platelet activation; cell migration; negative regulation of lipoprotein metabolic process; positive regulation of cell adhesion mediated by integrin; cell projection morphogenesis; angiogenesis involved in wound healing; cell-substrate adhesion; regulation of release of sequestered calcium ion into cytosol; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of osteoblast proliferation; activation of protein kinase activity; positive regulation of endothelial cell proliferation; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of cell migration