Mouse Bcl-2 homologous antagonist/killer Monoclonal Antibody | anti-BAK1 antibody

Mouse Anti Human Bcl-2 homologous antagonist/killer

Cat. Num. AAA140355

• Gene Names: BAK1; BAK; CDN1; BCL2L7; BAK-LIKE
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: BAK1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Bcl-2 homologous antagonist/killer; Monoclonal Antibody; Mouse Anti Human Bcl-2 homologous antagonist/killer; BAK1 Antibody; Mouse Anti Human; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK; BCL2L7; CDN1; MGC3887; BAK-LIKE; MGC117255; anti-BAK1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a heavy chain and k light chain
• Clone Number: PAT8B4AT
• Purity/Purification: BAK1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 211

• Applicable Applications for anti-BAK1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: BAK1 antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250.
• Immunogen: Anti-human BAK1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human BAK1 amino acids 29-187 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-BAK1 antibody

Introduction: BAK1 is a member of the BCL2 protein family. The BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators which are involved in a wide variety of cellular activities. The BAK1 gene encodes a receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 localizes to mitochondria, and functions to induce apoptosis. BAK1 interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, leading to a loss in membrane potential and the release of cytochrome c. Furthermore, BAK1 interacts with the tumor suppressor P53 after exposure to cell stress. BAK1 expression is linked to the progression of Prostate cancer.

Product Categories/Family for anti-BAK1 antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502363
• NCBI GeneID: 578
• NCBI Accession #: NP_001179.1
• NCBI GenBank Nucleotide #: NM_001188.3
• Molecular Weight: 16,872 Da
• NCBI Official Full Name: bcl-2 homologous antagonist/killer
• NCBI Official Synonym Full Names: BCL2-antagonist/killer 1
• NCBI Official Symbol: BAK1
• NCBI Official Synonym Symbols: BAK; CDN1; BCL2L7; BAK-LIKE
• NCBI Protein Information: bcl-2 homologous antagonist/killer; BCL2-like 7 protein; apoptosis regulator BAK; bcl-2-like protein 7; bcl2-L-7; pro-apoptotic protein BAK
• UniProt Protein Name: Bcl-2 homologous antagonist/killer
• Protein Family: Bcl-2 homologous antagonist/killer
• UniProt Gene Name: BAK1
• UniProt Synonym Gene Names: BAK; BCL2L7; CDN1; Bcl2-L-7
• UniProt Entry Name: BAK_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]

Uniprot Description

BAK1: In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. Interacts with BCL2A1. Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2, E1B 19k protein, and BCL2L1 isoform Bcl-X(L). Interacts with myxoma virus protein M11L. Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. Belongs to the Bcl-2 family.

Protein type: Apoptosis; Endoplasmic reticulum; Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: pore complex; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytosol; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity; metal ion binding; chaperone binding; heat shock protein binding

Biological Process: response to fungus; regulation of protein heterodimerization activity; positive regulation of proteolysis; apoptosis; positive regulation of apoptosis; regulation of cell cycle; myeloid cell homeostasis; negative regulation of peptidyl-serine phosphorylation; B cell apoptosis; response to organic cyclic substance; negative regulation of cell proliferation; B cell homeostasis; regulation of mitochondrial membrane potential; response to gamma radiation; establishment and/or maintenance of transmembrane electrochemical gradient; B cell negative selection; aging; response to drug; organ regeneration; release of cytochrome c from mitochondria; response to mycotoxin; mitochondrial fusion; regulation of protein homodimerization activity; vagina development; endocrine pancreas development; limb morphogenesis; response to UV-C; cell proliferation; endoplasmic reticulum calcium ion homeostasis; unfolded protein response, activation of signaling protein activity; response to ethanol; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; brain development; regulation of mitochondrial membrane permeability; caspase activation via cytochrome c; post-embryonic camera-type eye morphogenesis