Typical Testing Data/Standard Curve (for reference only)
Guinea Pig BAK1 ELISA Kit | BAK1 elisa kit
Guinea pig BAK1 (Bcl2 Antagonist/Killer 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BAK1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BAK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BAK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BAK1. You can calculate the concentration of BAK1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]
Uniprot Description
BAK1: In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. Interacts with BCL2A1. Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2, E1B 19k protein, and BCL2L1 isoform Bcl-X(L). Interacts with myxoma virus protein M11L. Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. Belongs to the Bcl-2 family.
Protein type: Mitochondrial; Endoplasmic reticulum; Membrane protein, integral; Apoptosis
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: pore complex; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytosol; integral to mitochondrial outer membrane
Molecular Function: identical protein binding; BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; chaperone binding; metal ion binding
Biological Process: response to fungus; regulation of protein heterodimerization activity; positive regulation of proteolysis; positive regulation of apoptosis; apoptosis; regulation of cell cycle; myeloid cell homeostasis; negative regulation of peptidyl-serine phosphorylation; B cell apoptosis; response to organic cyclic substance; negative regulation of cell proliferation; regulation of mitochondrial membrane potential; B cell homeostasis; response to gamma radiation; establishment and/or maintenance of transmembrane electrochemical gradient; B cell negative selection; aging; response to drug; release of cytochrome c from mitochondria; organ regeneration; mitochondrial fusion; response to mycotoxin; regulation of protein homodimerization activity; vagina development; endocrine pancreas development; limb morphogenesis; response to UV-C; cell proliferation; endoplasmic reticulum calcium ion homeostasis; response to ethanol; unfolded protein response, activation of signaling protein activity; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; regulation of mitochondrial membrane permeability; caspase activation via cytochrome c; brain development; post-embryonic camera-type eye morphogenesis
Research Articles on BAK1
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Product Notes
The Guinea Pig BAK1 bak1 (Catalog #AAA2501903) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2501903 ELISA Kit recognizes Guinea Pig BAK1. It is sometimes possible for the material contained within the vial of "BAK1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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