Mouse anti-Human, Mouse BBS5 Monoclonal Antibody | anti-BBS5 antibody

BBS5 Monoclonal Antibody

Cat. Num. AAA2541831

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Protein G purification
• Synonyms: BBS5; Monoclonal Antibody; BBS5 Monoclonal Antibody; Bardet Biedl syndrome 5 protein; Bardet-Biedl syndrome 5 protein; anti-BBS5 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 55
• Purity/Purification: Protein G purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 341

• Applicable Applications for anti-BBS5 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:5000; IHC: 1:50-1:500
• Immunogen: Fusion protein of BBS5
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, PH7.3
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Human brain tissue using BBS5 Monoclonal Antibody at dilution of 1:1000)

Related Product Information for anti-BBS5 antibody

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and identification is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.

Product Categories/Family for anti-BBS5 antibody

Metabolism; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 22748823
• NCBI GeneID: 129880
• NCBI Accession #: NP_689597.1
• NCBI GenBank Nucleotide #: NM_152384.2
• UniProt Accession #: Q8N3I7; Q6PKN0; D3DPC3
• Molecular Weight: Calculated: 39kDa; Observed: 39kDa
• NCBI Official Full Name: Bardet-Biedl syndrome 5 protein
• NCBI Official Synonym Full Names: Bardet-Biedl syndrome 5
• NCBI Official Symbol: BBS5
• NCBI Protein Information: Bardet-Biedl syndrome 5 protein
• UniProt Protein Name: Bardet-Biedl syndrome 5 protein
• Protein Family: Bardet-Biedl syndrome 5 protein
• UniProt Gene Name: BBS5

NCBI Description

This gene encodes a protein that has been directly linked to Bardet-Biedl syndrome. The primary features of this syndrome include retinal dystrophy, obesity, polydactyly, renal abnormalities and learning disabilities. Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.