Mouse Barmotin Monoclonal Antibody
Barmotin (Tight Junction-associated Protein)
Reactivity
Canine, Chicken, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Barmotin; Monoclonal Antibody; Barmotin (Tight Junction-associated Protein); Anti -Barmotin (Tight Junction-associated Protein); anti-Barmotin antibody
Host
Mouse
Reactivity
Canine, Chicken, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgM
Clone Number
7H6
Specificity
Recognizes human Barmotin at ~155kD. Localized at the periphery of tight junctions of hepatocytes and other epithelia and in the intestine and kidney. Species Crossreactivity: Canine, mouse, rat and chicken.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Applicable Applications for anti-Barmotin antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:10
Immunohistochemistry (Frozen): 1:10
Dilution: Western Blot: 1:10
Immunohistochemistry (Frozen): 1:10
Immunogen
Human Barmotin
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Barmotin antibody
Tight junctions function to maintain cellular polarity and permeability barriers in epithelial and endothelial cells. Several molecules of the tight junction complex have been identified. Studies with Madin-Darby canine kidney (MDCK) cells indicate the 7H6 antigen to be an important molecule for regulation of the barrier function of tight junctions. Confocal laser scanning microscopy revealed that the expression of 7H6 antigen decreased sequentially during hepatocarcinogenesis. The 7H6 antigen was expressed intensely at the apical and basolateral membrane of cancer cells with glandular pattern. Examination of human gastric and colon cancer tissues showed that tight junctions were maintained highly in the well-differentiated (gland-forming) adenocarcinomas, but reduced in poorly-differentiated adenocarcinomas. In MDCK cells the disappearance of 7H6 expression is closely related with cell spreading in vitro induced hepatocyte growth factor/scatter factor.
Product Categories/Family for anti-Barmotin antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
barmotin
Similar Products
Product Notes
The Barmotin (Catalog #AAA632414) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Barmotin (Tight Junction-associated Protein) reacts with Canine, Chicken, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Barmotin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:10 Immunohistochemistry (Frozen): 1:10. Researchers should empirically determine the suitability of the Barmotin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Barmotin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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