Mouse anti-Human SMAD4 Monoclonal Antibody | anti-SMAD4 antibody

SMAD4 (Pancreatic Adenocarcinoma Marker) Mouse Monoclonal Antibody [Clone SPM448]

Cat. Num. AAA4382066

• Gene Names: SMAD4; JIP; DPC4; MADH4; MYHRS
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: SMAD4; Monoclonal Antibody; SMAD4 (Pancreatic Adenocarcinoma Marker) Mouse Monoclonal Antibody [Clone SPM448]; SMAD4 (Pancreatic Adenocarcinoma Marker); Deleted in Pancreatic Carcinoma 4 (DPC4); JIP; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4 (MADH4); Mothers against DPP homolog 4; MYHRS; anti-SMAD4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2a, kappa
• Clone Number: SPM448
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-SMAD4 antibody: Immunohistochemistry-Formalin (IHC-F)
• Application Notes: IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT; (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH6.0, for 10-20 minutes followed by cooling at RT for 20 minutes)
• Immunogen: Recombinant full-length human STAT6 protein
• Cellular Localization: Cytoplasmic & Nuclear
• Positive Control: Jurkat or A431 cells. Pancreatic Carcinoma
• Hu-chromosome Location: 18q21.2
• Unigene: 75862
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)-Formalin

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with SMAD4 Mouse Monoclonal Antibody (SPM448). )

Related Product Information for anti-SMAD4 antibody

Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as DPC4. Upon ligand binding, the receptors of the TGF-beta family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. SMAD4 is absent in approximately 80% of pancreatic adenocarcinoma, but rarely in endometrial, colorectal, ovarian, lung, breast adenocarcinomas, and malignant melanom. SMAD4 is an important marker for confirming a identification of pancreatic adenocarcinoma. Patients with pancreatic adenocarcinomas with SMAD4 protein expression had significantly longer survival than SMAD4 negative patients.

NCBI and Uniprot Product Information

• NCBI GI #: 4885457
• NCBI GeneID: 4089
• NCBI Accession #: NP_005350.1
• NCBI GenBank Nucleotide #: NM_005359.5
• UniProt Accession #: Q13485; A8K405
• Molecular Weight: 60,439 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 4
• NCBI Official Synonym Full Names: SMAD family member 4
• NCBI Official Symbol: SMAD4
• NCBI Official Synonym Symbols: JIP; DPC4; MADH4; MYHRS
• NCBI Protein Information: mothers against decapentaplegic homolog 4; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4
• UniProt Protein Name: Mothers against decapentaplegic homolog 4
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD4
• UniProt Synonym Gene Names: DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4
• UniProt Entry Name: SMAD4_HUMAN

NCBI Description

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]

Uniprot Description

SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: nucleoplasm; centrosome; transcription factor complex; cytoplasm; nuclear chromatin; nucleus; cytosol

Molecular Function: collagen binding; identical protein binding; protein binding; protein homodimerization activity; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity; DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: developmental growth; axon guidance; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; sebaceous gland development; negative regulation of transcription from RNA polymerase II promoter; palate development; BMP signaling pathway; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; mesoderm development; neural crest cell differentiation; positive regulation of BMP signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor-beta2 production; transcription, DNA-dependent; regulation of binding; in utero embryonic development; neuron fate commitment; positive regulation of transforming growth factor beta receptor signaling pathway; gastrulation with mouth forming second; somite rostral/caudal axis specification; formation of anatomical boundary; SMAD protein complex assembly; endothelial cell activation; cell proliferation; ureteric bud branching; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of hair follicle development; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process; endoderm development

Disease: Pancreatic Cancer; Juvenile Polyposis Syndrome; Myhre Syndrome; Juvenile Polyposis/hereditary Hemorrhagic Telangiectasia Syndrome