Mouse anti-Human BACE1/ASP2 Monoclonal Antibody | anti-BACE1/ASP2 antibody

Mouse anti Human BACE1/ASP2 Monoclonal Antibody

Cat. Num. AAA2543226

• Gene Names: BACE1; ASP2; BACE; HSPC104
• Reactivity: Human
• Applications: ELISA
• Synonyms: BACE1/ASP2; Monoclonal Antibody; Mouse anti Human BACE1/ASP2 Monoclonal Antibody; anti-BACE1/ASP2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Sequence Length: 432

• Applicable Applications for anti-BACE1/ASP2 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect Human BACE1. The detection limit for Human BACE1 is approximately 0.31 ng/well.
• Immunogen: Recombinant Human BACE1 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 21040368
• NCBI GeneID: 23621
• NCBI GenBank Nucleotide #: NM_138973.3
• Molecular Weight: 42,107 Da
• NCBI Official Full Name: beta-secretase 1 isoform D
• NCBI Official Synonym Full Names: beta-secretase 1
• NCBI Official Symbol: BACE1
• NCBI Official Synonym Symbols: ASP2; BACE; HSPC104
• NCBI Protein Information: beta-secretase 1
• UniProt Protein Name: Beta-secretase 1
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2; Beta-site APP cleaving enzyme 1
• UniProt Entry Name: BACE1_HUMAN

NCBI Description

This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015]

Uniprot Description

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: EC 3.4.23.46; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: endoplasmic reticulum lumen; endosome; endosome membrane; Golgi apparatus; integral to plasma membrane; late endosome; multivesicular body; plasma membrane; trans-Golgi network

Molecular Function: aspartic-type endopeptidase activity; beta-amyloid binding; beta-aspartyl-peptidase activity; enzyme binding; peptidase activity; protein binding

Biological Process: beta-amyloid metabolic process; cellular protein metabolic process; membrane protein ectodomain proteolysis; protein catabolic process; proteolysis