Mouse anti-Porcine B Cell Subset Monoclonal Antibody
Mouse Anti-Porcine B Cell Subset-FITC
Reactivity
Porcine
Applications
Flow Cytometry, Immunohistochemistry
Synonyms
B Cell Subset; Monoclonal Antibody; Mouse Anti-Porcine B Cell Subset-FITC; Mouse Anti-Porcine B Cell Subset - FITC; Mouse Anti-Pig B Cell Subset; anti-B Cell Subset antibody
Host
Mouse
Reactivity
Porcine
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgM kappa
Clone Number
BB6-10A10
Specificity
Indeterminate
MAb BB6-10A10 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer's patches (ILPP); 6-17 in spleen; and 0% in thymus.1 While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by BB6-10A10 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIglow B cells in lymph nodes.
MAb BB6-10A10 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer's patches (ILPP); 6-17 in spleen; and 0% in thymus.1 While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by BB6-10A10 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIglow B cells in lymph nodes.
Form/Format
Fluorescein (FITC) Conjugate
Applicable Applications for anti-B Cell Subset antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
Application Notes
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Characterization
To ensure optimal performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
References
1. Denham, S., R.J. Zwart, J.T.D. Whittall, M. Pampusch, A.H. Corteyn, A.T.J. Bianchi, M.P. Murtaugh, R.M.E. Parkhouse, H. Tlaskalova, J. Sinkora, M. Siinkora, and Z. Rehakova. 1998. Monoclonal antibodies putatively identifying porcine B cells. Veter. Immunol. Immunopathol. 60:317-328.
2. Pescovitz, M. Personal communication.
2. Pescovitz, M. Personal communication.
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Product Notes
The B Cell Subset (Catalog #AAA673830) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Porcine B Cell Subset-FITC reacts with Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's B Cell Subset can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections). Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the B Cell Subset for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B Cell Subset, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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