Mouse ATPase, Ca Monoclonal Antibody
Anti-ATPase, Ca (Plasma Membrane, PMCA)
Reactivity
Canine; Chicken; Feline; Human; Monkey; Mouse; Rabbit; Rat
Purity
Ascites
Synonyms
ATPase; Ca; Monoclonal Antibody; Anti-ATPase; Ca (Plasma Membrane; PMCA); Plasma Membrane; PMCA; anti-ATPase; Ca antibody
Host
Mouse
Reactivity
Canine; Chicken; Feline; Human; Monkey; Mouse; Rabbit; Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3H5
Specificity
Crossreacts with human, chimpanzee, baboon, monkey, dog, rabbit, cat, rat, mouse, chicken, eel, plants and parasites (2
Purity/Purification
Ascites
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, 40% glycerol.
Concentration
1mg/ml (varies by lot)
Immunogen
Purified human erythrocyte Ca+-ATPases was used to immunize mice
Source Information
Human
Preparation and Storage
Store at -20 degree C.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for anti-ATPase, Ca antibody
Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein.
NCX contains a highly basic region in the large hydrophilic, intracellular loop called XIP (Exchange inhibitory peptide; RRLLFYKYVYKRYRAGKQRG 20 aa), that inhibits Na-Ca+ exchange in cardiac sarcolemmal vesicles and in other cells. Little or no sequence identity is found between the NCX and the Ca-pump. However, XIP also inhibits the Ca pumps with more or less same efficiency as C28R2 peptide sequence (LRRGQILWFRGLNRIQTQIRVVKAFRSS, 28 aa) corresponding to the autoinhibitory domain of Ca-pump.
Ca+-pump is a Mg+-dependent enzyme that catalyzes the hydrolysis of ATP with the transport of Ca+. Plasma membrane Ca+-ATPase (calcium pump) exists in several isoforms (human isofroms 1, 1084 aa; 1b, 1220 aa; from 2, 198/1243 aa; 3a/b 1173/1220 aa; 4, 1205 aa) and numerous sub-isoforms have been identified in various species. Ca+-pump displays 10 TM domains, with cytoplasmic N and C-termini. C28R2 is located in within the cytoplasmic, C-terminal domain of Ca+-ATPases.
NCX contains a highly basic region in the large hydrophilic, intracellular loop called XIP (Exchange inhibitory peptide; RRLLFYKYVYKRYRAGKQRG 20 aa), that inhibits Na-Ca+ exchange in cardiac sarcolemmal vesicles and in other cells. Little or no sequence identity is found between the NCX and the Ca-pump. However, XIP also inhibits the Ca pumps with more or less same efficiency as C28R2 peptide sequence (LRRGQILWFRGLNRIQTQIRVVKAFRSS, 28 aa) corresponding to the autoinhibitory domain of Ca-pump.
Ca+-pump is a Mg+-dependent enzyme that catalyzes the hydrolysis of ATP with the transport of Ca+. Plasma membrane Ca+-ATPase (calcium pump) exists in several isoforms (human isofroms 1, 1084 aa; 1b, 1220 aa; from 2, 198/1243 aa; 3a/b 1173/1220 aa; 4, 1205 aa) and numerous sub-isoforms have been identified in various species. Ca+-pump displays 10 TM domains, with cytoplasmic N and C-termini. C28R2 is located in within the cytoplasmic, C-terminal domain of Ca+-ATPases.
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Product Notes
The ATPase, Ca (Catalog #AAA632545) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ATPase, Ca (Plasma Membrane, PMCA) reacts with Canine; Chicken; Feline; Human; Monkey; Mouse; Rabbit; Rat and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "ATPase, Ca, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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