Mouse ATP7B Monoclonal Antibody | anti-ATP7B antibody

ATP7B (ATPase, Cu++ Transporting, beta Polypeptide, PWD, WC1, WD, WND) (HRP)

Cat. Num. AAA6181900

• Gene Names: ATP7B; WD; PWD; WC1; WND
• Applications: Western Blot
• Purity: Purified
• Synonyms: ATP7B; Monoclonal Antibody; ATP7B (ATPase; Cu++ Transporting; beta Polypeptide; PWD; WC1; WD; WND) (HRP); ATPase; WND; anti-ATP7B antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3A12
• Specificity: Recognizes ATP7B.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 1465

• Applicable Applications for anti-ATP7B antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: ATP7B (NP_000044, 1372aa-1465aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged ATP7B is approximately 0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-ATP7B antibody

Antibodies; Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 55743071
• NCBI GeneID: 540
• NCBI Accession #: NP_000044
• NCBI GenBank Nucleotide #: NP_000044.2
• UniProt Accession #: P35670
• NCBI Official Full Name: copper-transporting ATPase 2 isoform a
• NCBI Official Synonym Full Names: ATPase copper transporting beta
• NCBI Official Symbol: ATP7B
• NCBI Official Synonym Symbols: WD; PWD; WC1; WND
• NCBI Protein Information: copper-transporting ATPase 2
• UniProt Protein Name: Copper-transporting ATPase 2
• UniProt Gene Name: ATP7B
• UniProt Synonym Gene Names: PWD; WC1; WND
• UniProt Entry Name: ATP7B_HUMAN

NCBI Description

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein is a monomer, and functions as a copper-transporting ATPase which exports copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease which is characterized by copper accumulation. [provided by RefSeq, Dec 2019]

Uniprot Description

ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Vesicle; Membrane protein, integral; Transporter, ion channel; Hydrolase; Membrane protein, multi-pass; EC 3.6.3.54

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: Golgi membrane; membrane; mitochondrion; basolateral plasma membrane; perinuclear region of cytoplasm; integral to plasma membrane; cytoplasmic membrane-bound vesicle; late endosome; trans-Golgi network

Molecular Function: protein binding; copper ion binding; copper-exporting ATPase activity; ATP binding

Biological Process: lactation; cellular copper ion homeostasis; response to copper ion; metabolic process; cellular zinc ion homeostasis; sequestering of calcium ion; copper ion import; copper ion transport; copper ion export; transmembrane transport; intracellular copper ion transport

Disease: Wilson Disease