Typical Testing Data/Standard Curve (for reference only)
Human PLD2 (Phospholipase D2) CLIA Kit | PLD2 clia kit
Human PLD2 (Phospholipase D2) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PLD2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PLD2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PLD2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PLD2. You can calculate the concentration of PLD2 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Uniprot Description
PLD2: a phosphatidylcholine-specific phospholipase D. Catalyzes the hydrolysis of PC to produce phosphatidic acid and choline. Activated as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. May function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control. Enriched in caveolae; may be involved in MEK/ERK signaling cascades induced by the VEGF/VEGFR-2/PKC-delta pathway in endothelial cells. Three alternatively spliced isoforms have been described.
Protein type: Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis; Lipid Metabolism - ether lipid; Phospholipase; EC 3.1.4.4
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; brush border membrane; plasma membrane; vesicle
Molecular Function: protein binding; phospholipase D activity; phosphoinositide binding
Biological Process: exocytosis; phosphatidylglycerol biosynthetic process; phospholipid catabolic process; glycerophospholipid biosynthetic process; cell motility involved in cell locomotion; membrane lipid metabolic process; cytoskeleton organization and biogenesis; regulation of phagocytosis; regulation of cell migration; inositol lipid-mediated signaling; G-protein coupled receptor internalization; phosphatidic acid metabolic process; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidic acid biosynthetic process; innate immune response
Research Articles on PLD2
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Product Notes
The Human PLD2 pld2 (Catalog #AAA2531967) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531967 CLIA Kit recognizes Human PLD2. It is sometimes possible for the material contained within the vial of "PLD2 (Phospholipase D2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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