Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ARL5B protein (Right) or empty vector (Left) detected with ARL5B antibody)
Mouse ARL5B Monoclonal Antibody | anti-ARL5B antibody
ARL5B antibody
Gene Names
ARL5B; ARL8
Applications
Immunofluorescence, Western Blot
Purity
ARL5B antibody was purified by affinity chromatography.
Synonyms
ARL5B; Monoclonal Antibody; ARL5B antibody; Monoclonal ARL5B; Anti-ARL5B; ADP-ribosylation factor-like protein 5B; ARL8; ARLB 5; ARLB-5; anti-ARL5B antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2D7
Purity/Purification
ARL5B antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
179
Applicable Applications for anti-ARL5B antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100
WB: 1:2000
WB: 1:2000
Biological Significance
ADP-ribosylation factor-like protein 5B, also known as ARL5B, belongs to a family of proteins that are structurally similar to ADP-ribosylation factors. ARLs and ARFs are part of the RAS superfamily of regulatory GTPases. ARL5B is most closely related to ARL5, with which it shares 80% sequence identity. Human ARL5B shares 100% identity with mouse ARL8 and 71% identity with the Drosophila homolog. Two isoforms of ARL8 exist as a result of alternative splicing events. Recombinant human ARL5B protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Cross-Reactivity
Human
Immunogen
ARL5B antibody was raised in mouse using a full length recombinant protein of human ARL5B (NP_848930) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ARL5B protein (Right) or empty vector (Left) detected with ARL5B antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ARL5B protein (Right) or empty vector (Left) detected with ARL5B antibody)
Related Product Information for anti-ARL5B antibody
Mouse monoclonal ARL5B antibody
Product Categories/Family for anti-ARL5B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,375 Da
NCBI Official Full Name
ADP-ribosylation factor-like protein 5B
NCBI Official Synonym Full Names
ADP-ribosylation factor-like 5B
NCBI Official Symbol
ARL5B
NCBI Official Synonym Symbols
ARL8
NCBI Protein Information
ADP-ribosylation factor-like protein 5B
UniProt Protein Name
ADP-ribosylation factor-like protein 5B
Protein Family
UniProt Gene Name
ARL5B
UniProt Synonym Gene Names
ARL8
UniProt Entry Name
ARL5B_HUMAN
NCBI Description
ARL5B (ARL8) belongs to a family of proteins that are structurally similar to ADP-ribosylation factors (ARFs; see MIM 103180). ARLs and ARFs are part of the RAS superfamily of regulatory GTPases.[supplied by OMIM, Nov 2010]
Uniprot Description
ARL5B: Binds and exchanges GTP and GDP. Belongs to the small GTPase superfamily. Arf family.
Protein type: G protein, monomeric; G protein, monomeric, ARF
Chromosomal Location of Human Ortholog: 10p12.31
Cellular Component: intracellular
Molecular Function: GTP binding
Biological Process: small GTPase mediated signal transduction
Research Articles on ARL5B
Similar Products
Product Notes
The ARL5B arl5b (Catalog #AAA533554) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ARL5B can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the ARL5B arl5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARL5B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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