Immunohistochemistry (IHC)
(Anti-ARHGAP25 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Mouse anti-Human ARHGAP25 Monoclonal Antibody | anti-ARHGAP25 antibody
Anti-ARHGAP25 Antibody (clone 12G4) IHC-plus
IF (1:100)
IHC-P (10 ug/ml)
WB (1:500 - 1:2000)
Immunohistochemistry (IHC)
(Anti-ARHGAP25 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-ARHGAP25 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunofluorescence (IF)
(Anti-ARHGAP25 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARHGAP25.)
Immunofluorescence (IF)
(Anti-ARHGAP25 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARHGAP25.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25.)
Western Blot (WB)
(Western blot of extracts (35 ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.)
Western Blot (WB)
(Western blot of extracts (35 ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARHGAP25 antibody, and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARHGAP25 antibody, and then analyzed by flow cytometry.)
NCBI and Uniprot Product Information
NCBI Description
ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
Uniprot Description
ARHGAP25: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: GAPs; GAPs, Rac/Rho
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: cytosol
Molecular Function: GTPase activator activity
Biological Process: positive regulation of GTPase activity; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction
Research Articles on ARHGAP25
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Product Notes
The ARHGAP25 arhgap25 (Catalog #AAA249397) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARHGAP25 Antibody (clone 12G4) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP25 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS). Flow Cytometry (1:100) IF (1:100) IHC-P (10 ug/ml) WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the ARHGAP25 arhgap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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