Mouse anti-Human Arginase-I Monoclonal Antibody | anti-Arg1 antibody

Arginase-I (Liver Type) (EC 3.5.3.1, L-arginine aminohydrolase)

Cat. Num. AAA603405

• Gene Names: Arg1; AI; PGIF; Arg-1; AI256583
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Arginase-I; Monoclonal Antibody; Arginase-I (Liver Type) (EC 3.5.3.1; L-arginine aminohydrolase); Anti -Arginase-I (Liver Type) (EC 3.5.3.1; anti-Arg1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5i139
• Specificity: Recognizes human liver-type aginase.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-Arg1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Recombinant human liver-type aginase from E. coli.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Arg1 antibody

Arginase is an enzyme that hydrolyzes Larginine to L-ornithine and urea in the urea cycle. Two forms of arginase exists which are designed as arginase I and arginase II. Liver-type arginase I is expressed primarily in the liver and to some extend in the erythrocytes. Arginase II is expressed in many extrahepatic tissues, such as brain, spinal cord, kidney, small intestine and mammary gland. Although arginase I and arginase II have similar enzyme activities, they have different pI, immunological reactivity and are encoded by different genes. Human arginase I is a 35kD protein circulating in blood probably as a homotrimer. Circulating liver-type arginase was clinically used as a liver specific marker which may reflect not only early occurrence of liver injury but also early termination of liver injury. The measurement of liver-type arginase is clinically applicable for monitoring conditions of patients with liver disorders or pre- and postoperative conditions of patients who received partial hepatectomy with quicker normalization in comparison with aminotransferases (ALT and AST). Recently, arginase I gene was found to be one of the most prominent among astma genes. In situ hybridization demonstrated marked staining of arginase I in submucosal inflammatory lesions and arginase activity increased in allergen challenged lungs. Finally, it was found that both arginase I was the most significantly up-regulated protein in the murine spinal cord during experimental autoimmune encephalomyelitis. The results indicated that arginase I played important roles in autoimmune inflammation in the central nervous system.

Product Categories/Family for anti-Arg1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 7106255
• NCBI GeneID: 11846
• NCBI Accession #: NP_031508.1
• NCBI GenBank Nucleotide #: NM_007482.3
• UniProt Accession #: Q61176; Q3TB74; Q3UEL0; Q4FK78; Q80VI4; Q8K3K5
• Molecular Weight: 34,808 Da
• NCBI Official Full Name: arginase-1
• NCBI Official Synonym Full Names: arginase, liver
• NCBI Official Symbol: Arg1
• NCBI Official Synonym Symbols: AI; PGIF; Arg-1; AI256583
• NCBI Protein Information: arginase-1; arginase I; type I arginase; arginase 1, liver; liver-type arginase
• UniProt Protein Name: Arginase-1
• Protein Family: Arginase
• UniProt Gene Name: Arg1
• UniProt Entry Name: ARGI1_MOUSE

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Amino Acid Metabolism - arginine and proline; EC 3.5.3.1

Cellular Component: extracellular space; mitochondrial outer membrane; neuron projection; cell soma; cytoplasm; nucleus

Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines; hydrolase activity; metal ion binding; manganese ion binding; arginase activity

Biological Process: arginine metabolic process; arginine catabolic process to ornithine; positive regulation of endothelial cell proliferation; collagen biosynthetic process; urea cycle