Mouse anti-Bovine Aprotinin Monoclonal Antibody

Aprotinin (Pancreatic trypsin inhibitor)

Cat. Num. AAA602898

• Reactivity: Bovine
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: Aprotinin; Monoclonal Antibody; Aprotinin (Pancreatic trypsin inhibitor); Anti -Aprotinin (Pancreatic trypsin inhibitor); anti-Aprotinin antibody

• Host: Mouse
• Reactivity: Bovine
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: 9A509
• Specificity: Binds bovine aprotinin immobilized on a solid phase or free in solution. Epitope is different from A2300-01 and A2300-01C.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide before the addition of glycerol to 40%.

• Applicable Applications for anti-Aprotinin antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA.; Dilution: ELISA: 1:33,000. 1ug/ml A2300-01A when biotinylated (or A2300-01B), forms an antibody pair with A2300-01C (as capture antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20ug/ml. Aprotinin in urine diluted 1:1000 can be assayed against standards in buffer. For assay in plasma, it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution.; A2300-01A when biotinylated (or A2300-01B), also forms an antibody pair with A2300-01 (as capture antibody) for sandwich ELISA of aprotinin at samples concentrations of 4-250ug/ml. Both urine and plasma samples diluted 1;1000 can be assayed against standards in buffer. PBS-based buffers are unsuitable for this assay and 0.2M glycine sodium buffer, pH 8.8, is recommended.
• Immunogen: Aprotinin from bovine lung adsorbed onto aluminum hydroxide gel.
• Culture Medium: RPMI 1640 with 2-10% fetal calf serum.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Aprotinin antibody

Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury (1).

Product Categories/Family for anti-Aprotinin antibody

Antibodies; Abs to Protease Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 58005
• NCBI Official Full Name: aprotinin