anti-Human Apolipoprotein AI (Apo AI) Monoclonal Antibody | anti-APOA1 antibody

MAb to Apolipoprotein AI

Cat. Num. AAA311600

• Gene Names: APOA1; MGC117399
• Reactivity: Human
• Purity: >90% pure (SDS-PAGE). Protein A chromatography; Product is 0.2um filtered.
• Synonyms: Apolipoprotein AI (Apo AI); Monoclonal Antibody; MAb to Apolipoprotein AI; Monoclonal Antibody to Human Apolipoprotein AI (Apo AI); anti-APOA1 antibody

• Host: Host: Mouse; Source: Ascites
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: 057-10029
• Specificity: Apolipoprotein AI (Apo AI); This product does not cross react with Apo AII or Apo B in competitive RIA.
• Purity/Purification: >90% pure (SDS-PAGE). Protein A chromatography; Product is 0.2um filtered.
• Form/Format: Purified, Liquid
• Concentration: 5.3mg/ml (OD280nm, E^0.1% = 1.4) (varies by lot)
• Sequence Length: 267

• Immunogen: Human high density lipoprotein
• Affinity Constant: 5 x 10^6 L/m (determined by RIA followed by Scatchard plot analysis)
• Buffer: 10mM Phosphate, pH 7.4 containing 150mM Sodium chloride
• Preservative: 0.1% Sodium azide
• Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Preparation and Storage: Short term (up to 7 days) store at 2 to 8 degree C. Long term, aliquot and store at <-40 degree C. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used. Avoid repeated freeze/th

Product Categories/Family for anti-APOA1 antibody

Monoclonal Antibodies to Lipoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 296635
• NCBI GeneID: 335
• UniProt Accession #: P02647; Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8; A8K866; Q6LEJ8; Q8TDB0; Q9Y355
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein AI
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Official Synonym Symbols: MGC117399
• NCBI Protein Information: apolipoprotein A-I; apo-AI; apoA-I; OTTHUMP00000043268; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Entry Name: APOA1_HUMAN

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]

Uniprot Description

Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Ref.20

Subunit structure: Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Ref.25 Ref.31

Subcellular location: Secreted.

Tissue specificity: Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. Ref.33

Post-translational modification: Palmitoylated. Ref.28Phosphorylation sites are present in the extracelllular medium.

Involvement in disease: Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [

MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant. Ref.37 Ref.38Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [

MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I. Ref.37 Ref.38Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [

MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed. Ref.37 Ref.38 Ref.43 Ref.44Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [

MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Ref.37 Ref.38 Ref.46

Sequence similarities: Belongs to the apolipoprotein A1/A4/E family.