anti-Human, Mouse AS160 Antibody | anti-AS160 antibody

AS160 (Ab-642) Antibody

Cat. Num. AAA8509430

• Gene Names: TBC1D4; AS160; NIDDM5
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: AS160; AS160 (Ab-642) Antibody; Akt substrate of 160 kDa; KIAA0603; TBC1 domain; anti-AS160 antibody

• Reactivity: Human, Mouse
• Specificity: AS160 (Ab-642) antibody detects endogenous levels of total AS160 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1290

• Applicable Applications for anti-AS160 antibody: Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human AS160 around the phosphorylation site of threonine 642 (A-H-TP-F-S).
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of HeLa cells, using AS160 (Ab-642) antibody.)

References

Nagase T., DNA Res. 5:31-39(1998). Dunham A., Nature 428:522-528(2004). Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 557786188
• NCBI GeneID: 9882
• NCBI Accession #: NP_001273587.1
• NCBI GenBank Nucleotide #: NM_001286658.2
• UniProt Accession #: O60343; Q5W0B9; Q68D14; A7E2X8; B4DU25; B4E235; B6ETN8; B6ETN9
• Molecular Weight: 60,095 Da
• NCBI Official Full Name: TBC1 domain family member 4 isoform 2
• NCBI Official Synonym Full Names: TBC1 domain family member 4
• NCBI Official Symbol: TBC1D4
• NCBI Official Synonym Symbols: AS160; NIDDM5
• NCBI Protein Information: TBC1 domain family member 4
• UniProt Protein Name: TBC1 domain family member 4
• UniProt Gene Name: TBC1D4
• UniProt Synonym Gene Names: AS160; KIAA0603; AS160

NCBI Description

This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.