Standard Curve (Sample)
Rat Calcium/calmodulin-dependent protein kinase II inhibitor 1 (CAMK2N1) ELISA Kit | CAMK2N1 elisa kit
Rat Calcium/calmodulin-dependent protein kinase II inhibitor 1 (CAMK2N1) ELISA Kit
Gene Names
CAMK2N1; PRO1489
Reactivity
Rat
Synonyms
Calcium/calmodulin-dependent protein kinase II inhibitor 1 (CAMK2N1); N/A; Rat Calcium/calmodulin-dependent protein kinase II inhibitor 1 (CAMK2N1) ELISA Kit; CAMK2N1 elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
10-250ng/mL
Sensitivity
1.0ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CAMK2N1 elisa kit
Intended Uses: This CAMK2N1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CAMK2N1. This ELISA kit for research use only!
Principle of the Assay||CAMK2N1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CAMK2N1 antibody and an CAMK2N1-HRP conjugate. The assay sample and buffer are incubated together with CAMK2N1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMK2N1 concentration since CAMK2N1 from samples and CAMK2N1-HRP conjugate compete for the anti-CAMK2N1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMK2N1 from the sample, fewer sites are left to bind CAMK2N1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK2N1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||CAMK2N1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CAMK2N1 antibody and an CAMK2N1-HRP conjugate. The assay sample and buffer are incubated together with CAMK2N1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMK2N1 concentration since CAMK2N1 from samples and CAMK2N1-HRP conjugate compete for the anti-CAMK2N1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMK2N1 from the sample, fewer sites are left to bind CAMK2N1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK2N1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8,553 Da
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase II inhibitor 1
NCBI Official Synonym Full Names
calcium/calmodulin-dependent protein kinase II inhibitor 1
NCBI Official Symbol
CAMK2N1
NCBI Official Synonym Symbols
PRO1489
NCBI Protein Information
calcium/calmodulin-dependent protein kinase II inhibitor 1; CaMKIINalpha; caMKIIN-alpha; caMKII inhibitory protein alpha
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase II inhibitor 1
UniProt Gene Name
CAMK2N1
UniProt Synonym Gene Names
CaMKIIN-alpha
UniProt Entry Name
CK2N1_HUMAN
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Product Notes
The Rat CAMK2N1 camk2n1 (Catalog #AAA246664) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA246664 ELISA Kit recognizes Rat CAMK2N1. It is sometimes possible for the material contained within the vial of "Calcium/calmodulin-dependent protein kinase II inhibitor 1 (CAMK2N1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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