Mouse anti-Human APOA5 Monoclonal Antibody | anti-APOA5 antibody

APOA5 (Apolipoprotein A5, Apo A-V, Apolipoprotein A-V, ApoA-V)

Cat. Num. AAA603509

• Gene Names: APOA5; RAP3; APOAV; FLJ97995; MGC126836; MGC126838
• Reactivity: Human
• Applications: ELISA
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: APOA5; Monoclonal Antibody; APOA5 (Apolipoprotein A5; Apo A-V; Apolipoprotein A-V; ApoA-V); Anti -APOA5 (Apolipoprotein A5; anti-APOA5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 8J289
• Specificity: Recognizes recombinant Apolipoprotein A5 protein and human serum.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid

• Applicable Applications for anti-APOA5 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Immunogen: Ni-NTA purified full length recombinant human APOA5 expressed in E. Coli strain BL21 (DE3).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-APOA5 antibody

Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor-4 (PPAR-alpha) agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Recently, it was demonstrated that ApoA5 is present in human serum detected by polyclonal antibodies against both the NH2 and COOH termini, although at much lower concentration than other apolipoproteins.

Product Categories/Family for anti-APOA5 antibody

Antibodies; Abs to Lipoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 262231737
• NCBI GeneID: 116519
• NCBI Accession #: NP_001160070.1
• NCBI GenBank Nucleotide #: NM_001166598.1
• UniProt Accession #: Q6Q788; Q3MIK6; Q6UWK9; Q9UBJ3; B0YIV9
• Molecular Weight: 41,213 Da
• NCBI Official Full Name: apolipoprotein A-V
• NCBI Official Synonym Full Names: apolipoprotein A-V
• NCBI Official Symbol: APOA5
• NCBI Official Synonym Symbols: RAP3; APOAV; FLJ97995; MGC126836; MGC126838
• NCBI Protein Information: apolipoprotein A-V; apo-AV; apolipoprotein A5; OTTHUMP00000043271; OTTHUMP00000069351; regeneration-associated protein 3
• UniProt Protein Name: Apolipoprotein A-V
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA5
• UniProt Synonym Gene Names: RAP3
• UniProt Entry Name: APOA5_HUMAN

NCBI Description

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]

Uniprot Description

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Lipid-binding; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; extracellular region

Molecular Function: heparin binding; enzyme binding; low-density lipoprotein receptor binding; cholesterol transporter activity; enzyme activator activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding

Biological Process: positive regulation of fatty acid biosynthetic process; organ regeneration; response to hormone stimulus; neurite regeneration; positive regulation of lipid catabolic process; lipoprotein metabolic process; cholesterol efflux; cellular lipid metabolic process; lipid transport; cholesterol biosynthetic process; positive regulation of receptor-mediated endocytosis; phosphatidylcholine metabolic process; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; regulation of cholesterol absorption; phospholipid efflux; acylglycerol homeostasis; tissue regeneration; triacylglycerol catabolic process; positive regulation of lipoprotein lipase activity

Disease: Hypertriglyceridemia, Familial; Hyperlipoproteinemia, Type V