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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANXA11 protein (Right) or empty vector (Left) detected with ANXA11 antibody)

Mouse ANXA11 Monoclonal Antibody | anti-ANXA11 antibody

ANXA11 antibody

Gene Names
ANXA11; ANX11; CAP50
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
ANXA11 antibody was purified by affinity chromatography.
Synonyms
ANXA11; Monoclonal Antibody; ANXA11 antibody; Monoclonal ANXA11; Anti-ANXA11; A11; ANX11; CAP50; Annexin-11; Annexin XI; ANXA 11; ANXA-11; anti-ANXA11 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1C6
Purity/Purification
ANXA11 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
505
Applicable Applications for anti-ANXA11 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:2000
Biological Significance
ANXA11 (Annexin A11) is a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. ANXA11 binds specifically to calcyclin in a calcium-dependent manner. And it required for midbody formation and completion of the terminal phase of cytokinesis. This protein is a 56-kD protein recognized by sera from patients with various autoimmune diseases.
Cross-Reactivity
Human
Immunogen
ANXA11 antibody was raised in mouse using a full length recombinant protein of human ANXA11 (NP_001148) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANXA11 protein (Right) or empty vector (Left) detected with ANXA11 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANXA11 protein (Right) or empty vector (Left) detected with ANXA11 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANXA11 protein using ANXA11 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANXA11 protein using ANXA11 antibody)
Related Product Information for anti-ANXA11 antibody
Mouse monoclonal ANXA11 antibody
Product Categories/Family for anti-ANXA11 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
311
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,242 Da
NCBI Official Full Name
annexin A11 isoform 1
NCBI Official Synonym Full Names
annexin A11
NCBI Official Symbol
ANXA11
NCBI Official Synonym Symbols
ANX11; CAP50
NCBI Protein Information
annexin A11
UniProt Protein Name
Annexin A11
Protein Family
UniProt Gene Name
ANXA11
UniProt Synonym Gene Names
ANX11; CAP-50
UniProt Entry Name
ANX11_HUMAN

NCBI Description

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified. [provided by RefSeq, May 2013]

Uniprot Description

ANXA11: a calcium/phospholipid-binding protein found throughout the nucleoplasm at interphase and at the mitotic phase is concentrated at the loop-like structure around the mitotic apparatus. May interact with calcyclin (S100A6) in vivo. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Nuclear envelope; Lipid-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleoplasm; specific granule; azurophil granule; membrane; cytoplasm; melanosome; spindle; nuclear envelope; midbody; phagocytic vesicle

Molecular Function: protein binding; phosphatidylethanolamine binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding

Biological Process: response to calcium ion; phagocytosis

Research Articles on ANXA11

Similar Products

Product Notes

The ANXA11 anxa11 (Catalog #AAA831386) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ANXA11 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the ANXA11 anxa11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANXA11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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