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Western Blot (WB)
(Western Blot detection against Immunogen (63.8kD).)
Mouse anti-Human ANXA1 Monoclonal Antibody | anti-ANXA1 antibody
ANXA1 (Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 Inhibitory Protein, p35, ANX1, LPC1) (AP)
Gene Names
ANXA1; ANX1; LPC1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ANXA1; Monoclonal Antibody; ANXA1 (Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 Inhibitory Protein; p35; ANX1; LPC1) (AP); anti-ANXA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2D11
Specificity
Recognizes human ANXA1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-ANXA1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-346 from human ANXA1 (AAH01275) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (63.8kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (63.8kD).)
Western Blot (WB)
(ANXA1 monoclonal antibody. Western Blot analysis of ANXA1 expression in human placenta.)
Western Blot (WB)
(ANXA1 monoclonal antibody. Western Blot analysis of ANXA1 expression in human placenta.)
Western Blot (WB)
(ANXA1 monoclonal antibody. Western Blot analysis of ANXA1 expression in HepG2.)
Western Blot (WB)
(ANXA1 monoclonal antibody. Western Blot analysis of ANXA1 expression in HepG2.)
Western Blot (WB)
(Western Blot analysis of ANXA1 expression in transfected 293T cell line by ANXA1 monoclonal antibody. Lane 1: ANXA1 transfected lysate (38.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ANXA1 expression in transfected 293T cell line by ANXA1 monoclonal antibody. Lane 1: ANXA1 transfected lysate (38.7kD). Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged ANXA1 is 3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged ANXA1 is 3ng/ml as a capture antibody.)
Related Product Information for anti-ANXA1 antibody
The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology. Annexin-1 (ANXA1) is the frst characterized member of the annexin family of proteins able to bind to cellular membranes in a calcium-dependent manner which promotes membrane fusion and is involved in endocytosis. Annexin A1 has anti-infammatory properties and inhibits phospholipase A2 activity. Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis,suggesting that it may be required for a late stage in inward vesiculation.Phosphorylation by PKC, EGFR and Chak1 results in inhibition of annexin A1 function. Annexin A1 has also been identifed as one of the 'eat-me' signals on apoptotic cells to be recognised and ingested by phagocytes. Annexin A1, as an endogenous anti-infammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, infammation, phagocytosis,proliferation, apoptosis, and tumorigenesis and cancer development.
Product Categories/Family for anti-ANXA1 antibody
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
Research Articles on ANXA1
Similar Products
Product Notes
The ANXA1 (Catalog #AAA6130003) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ANXA1 (Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 Inhibitory Protein, p35, ANX1, LPC1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ANXA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ANXA1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANXA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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