anti-Human SP100 Antibody | anti-SP100 antibody

SP100 Antibody

Cat. Num. AAA858017

• Gene Names: SP100; lysp100b
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: SP100; SP100 Antibody; DKFZp686E07254; FLJ00340; FLJ34579; Lysp100b; nuclear antigen Sp100; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; SP100 nuclear antigen; SP100-HMG nuclear autoantigen; Speckled 100 kDa; anti-SP100 antibody

• Reactivity: Human
• Specificity: SP100 antibody detects endogenous levels of total SP100protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 885

• Applicable Applications for anti-SP100 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from Internal of human SP100.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

Product Categories/Family for anti-SP100 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Dent A.L., Blood 88:1423-1426(1996). Seeler J.-S., Proc. Natl. Acad. Sci. U.S.A. 95:7316-7321(1998). Szostecki C., J. Immunol. 145:4338-4347(1990).

NCBI and Uniprot Product Information

• NCBI GI #: 122939208
• NCBI GeneID: 6672
• NCBI Accession #: NP_001073860.1
• NCBI GenBank Nucleotide #: NM_001080391.1
• UniProt Accession #: P23497; O75450; Q13343; Q8TE34; Q96F70; Q96T24; Q96T95; B4DDX5; B8ZZD8; E7EUA7; E9PH61; F8WFE2
• Molecular Weight: 50,032 Da
• NCBI Official Full Name: nuclear autoantigen Sp-100 isoform 1
• NCBI Official Synonym Full Names: SP100 nuclear antigen
• NCBI Official Symbol: SP100
• NCBI Official Synonym Symbols: lysp100b
• NCBI Protein Information: nuclear autoantigen Sp-100
• UniProt Protein Name: Nuclear autoantigen Sp-100
• Protein Family: Nuclear autoantigen
• UniProt Gene Name: SP100

NCBI Description

This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]

Uniprot Description

Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to PubMed:11909962. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to PubMed:15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation.