Mouse ANTHRAX Monoclonal Antibody | anti-pxo1_110 antibody

MOUSE ANTI ANTHRAX PROTECTIVE ANTIGEN

Cat. Num. AAA214545

• Applications: ELISA, Western Blot
• Synonyms: ANTHRAX; Monoclonal Antibody; MOUSE ANTI ANTHRAX PROTECTIVE ANTIGEN; anti-pxo1_110 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C3
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 764

• Applicable Applications for anti-pxo1_110 antibody: ELISA (EIA), Western Blot (WB)
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Highly purified protective antigen of Bacillus anthracis.; Fusion Partners
• Target Species: Bacterial
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-pxo1_110 antibody

Mouse anti Anthrax Protective Antigen antibody, clone C3 recognizes the Protective Antigen of the anthrax toxin. Bacillus anthracis produces a toxin composed of three proteins, the Protective Antigen (PA), the Edema Factor (EF) and the Lethal Factor (LF). The three proteins depend on each other for their toxic effect. Both EF and LF functions intracellularly but are too large to enter the cell through existing channels. The PA is responsible for transporting the EF and LF into cells. The 83kDa PA (PA83) binds to the cell surface where a 20 kDa N-terminal fragment (PA20) is cleaved off, exposing binding sites for LF and EF. The remaining 63 kDa portion (PA63) oligomerizes to form a heptamer pre-pore, which then associates with up to three molecules of EF and/or LF. The receptor complex is internalized within endocytotic vessels that are subsequently acidified, transforming the PA heptamer into a pore that releases the active enzymes EF (an adenylate cyclase) and LF (a protease targeting MAPKKs) into the cytosol. Mouse anti Anthrax Protective Antigen antibody, clone C3 does not cross-react with the Lethal Factor of Bacillus anthracis, Yersinia pestis, Francisella tularensis or Toxoplasma gondii. The antibody recognizes both the 83kDa and 63kDa truncated form of PA.

NCBI and Uniprot Product Information

• NCBI GI #: 10956357
• NCBI GeneID: 3361714
• NCBI Accession #: NP_052806.1
• NCBI GenBank Nucleotide #: NC_001496.1
• UniProt Accession #: P13423; Q937W2; Q937W3; Q9F5R7; Q9KH69; Q9RQU2; F59104
• Molecular Weight: 85,811 Da
• NCBI Official Full Name: pXO1-110
• NCBI Official Symbol: pxo1_110
• NCBI Protein Information: pXO1-110
• UniProt Protein Name: Protective antigen
• Protein Family: Anthrax toxin receptor
• UniProt Gene Name: pagA
• UniProt Synonym Gene Names: pag; PA; PA83; PA20; PA63
• UniProt Entry Name: PAG_BACAN

Uniprot Description

One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.