Mouse anti-Human Annexin A1 Monoclonal Antibody | anti-ANXA1 antibody

Annexin A1 Antibody

Cat. Num. AAA346001

• Gene Names: ANXA1; ANX1; LPC1
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: Annexin A1; Monoclonal Antibody; Annexin A1 Antibody; anti-ANXA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: IHC512
• Form/Format: Liquid
• Sequence Length: 346

• Applicable Applications for anti-ANXA1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Dilution Range: 1:100 - 1:200
• Buffer: Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
• IHC Positive Control: Hairy Cell Leukemia
• IHC Suggested Protocol: 1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.; 2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.; 3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.; 4. Block – Block with peroxidase solution for 5 minutes at RT.; 5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.; 6. Secondary Ab - Incubate for 20 - 30 minutes at RT.; 7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.; 8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.; 9. Dehydrate and apply coverslip.; (Each researcher should optimize the protocol for their experimental model system.)
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Immunohistochemistry (IHC)

(IHC (paraffin) of human spleen using Annexin A1 Antibody)

Related Product Information for anti-ANXA1 antibody

Annexin A1 is a member of the annexin family, a group of phospholipid-binding membrane proteins that plays a role in both the innate and adaptive immune system. Its plays an important role in controlling inflammation, prostaglandins, and leukotriene mediators. Annexin A1 is overexpressed in 97% of hairy cell leukemia samples, and is absent in other B cell lymphomas.

NCBI and Uniprot Product Information

• NCBI GI #: 4502101
• NCBI GeneID: 301
• NCBI Accession #: NP_000691.1
• NCBI GenBank Nucleotide #: NM_000700.2
• UniProt Accession #: P04083
• Molecular Weight: 38,714 Da
• NCBI Official Full Name: annexin A1
• NCBI Official Synonym Full Names: annexin A1
• NCBI Official Symbol: ANXA1
• NCBI Official Synonym Symbols: ANX1; LPC1
• NCBI Protein Information: annexin A1
• UniProt Protein Name: Annexin A1
• Protein Family: Annexin
• UniProt Gene Name: ANXA1
• UniProt Synonym Gene Names: ANX1; LPC1

NCBI Description

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]

Uniprot Description

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (). Promotes resolution of inflammation and wound healing (PubMed:25664854). Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca2+ and can bind up to eight Ca2+ ions (). Displays Ca2+-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca2+-dependent interaction between phagosomes and the actin cytoskeleton ().