Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANKRD53 protein (Right) or empty vector (Left) detected with ANKRD53 antibody)
Mouse ANKRD53 Monoclonal Antibody | anti-ANKRD53 antibody
ANKRD53 antibody
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
ANKRD53 antibody was purified by affinity chromatography.
Synonyms
ANKRD53; Monoclonal Antibody; ANKRD53 antibody; Monoclonal ANKRD53; Anti-ANKRD53; Ankyrin repeat domain 53; FLJ12056; OTTHUMP00000203459; Ankyrin repeat domain-containing protein 53; ANKRD 53; ANKRD-53; anti-ANKRD53 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1C10
Purity/Purification
ANKRD53 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
343
Applicable Applications for anti-ANKRD53 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:2000
IF: 1:100
WB: 1:2000
Biological Significance
Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.
Cross-Reactivity
Human
Immunogen
ANKRD53 antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 1-300 of human ANKRD53 (NP_079209) produced in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANKRD53 protein (Right) or empty vector (Left) detected with ANKRD53 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANKRD53 protein (Right) or empty vector (Left) detected with ANKRD53 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANKRD53 protein using ANKRD53 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANKRD53 protein using ANKRD53 antibody)
Related Product Information for anti-ANKRD53 antibody
Mouse monoclonal ANKRD53 antibody
Product Categories/Family for anti-ANKRD53 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,200 Da
NCBI Official Full Name
ankyrin repeat domain-containing protein 53 isoform b
NCBI Official Synonym Full Names
ankyrin repeat domain 53
NCBI Official Symbol
ANKRD53
NCBI Protein Information
ankyrin repeat domain-containing protein 53
UniProt Protein Name
Ankyrin repeat domain-containing protein 53
Protein Family
UniProt Gene Name
ANKRD53
UniProt Entry Name
ANR53_HUMAN
Uniprot Description
ANKRD53: 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 2p13.3
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Product Notes
The ANKRD53 ankrd53 (Catalog #AAA830990) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ANKRD53 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the ANKRD53 ankrd53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANKRD53, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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