Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANAPC2 protein (Right) or empty vector (Left) detected with ANAPC2 antibody)
Mouse ANAPC2 Monoclonal Antibody | anti-ANAPC2 antibody
ANAPC2 antibody
FC: 1:100
IHC: 1:150
WB: 1:500-2000
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANAPC2 protein (Right) or empty vector (Left) detected with ANAPC2 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANAPC2 protein (Right) or empty vector (Left) detected with ANAPC2 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of ANAPC2 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using ANAPC2 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of ANAPC2 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using ANAPC2 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANAPC2 protein using ANAPC2 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANAPC2 protein using ANAPC2 antibody)
NCBI and Uniprot Product Information
NCBI Description
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008]
Uniprot Description
APC2: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the cullin family. The APC/C is composed of at least 12 subunits. Interacts through the cullin domain with ANAPC11 and with UBCH10. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol
Molecular Function: ubiquitin protein ligase binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of axon extension; mitotic cell cycle spindle assembly checkpoint; positive regulation of synaptic plasticity; mitotic cell cycle; positive regulation of dendrite morphogenesis
Research Articles on ANAPC2
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Product Notes
The ANAPC2 anapc2 (Catalog #AAA831802) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ANAPC2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 FC: 1:100 IHC: 1:150 WB: 1:500-2000. Researchers should empirically determine the suitability of the ANAPC2 anapc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANAPC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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