Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunohistochemistry (IHC) (Anti-AMY1A / Salivary Amylase antibody IHC of human salivary gland. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Mouse anti-Human AMY1A / Salivary Amylase Monoclonal Antibody | anti-AMY1A antibody

Mouse Monoclonal [clone 2D4] (IgG2a,k) to Human AMY1A / Salivary Amylase

Gene Names
AMY1A; AMY1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
AMY1A / Salivary Amylase; Monoclonal Antibody; Mouse Monoclonal [clone 2D4] (IgG2a; k) to Human AMY1A / Salivary Amylase; Anti-AMY1A / Salivary Amylase Antibody (clone 2D4) IHC-plus; Alpha-amylase 1; Glycogenase; AMY1; Amylase; alpha 1A (salivary); alpha 1A salivary; salivary; alpha-1A; Salivary amylase alpha 1A; Salivary alpha-amylase; Salivary Amylase; Human AMY1A; anti-AMY1A antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2D4
Specificity
Human AMY1A
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Concentration
0.45 mg/ml (varies by lot)
Sequence Length
511
Applicable Applications for anti-AMY1A antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Tissue lysate, Recombinant protein.
Target Species
Human
Immunogen Description
AMY1A (NP_001008222, 172 a.a. ~ 246 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
AMY1A / Salivary Amylase antibody was raised against aMY1A (NP_001008222, 172 a.a. ~ 246 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Aliquot and freeze at -20 degree C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-AMY1A / Salivary Amylase antibody IHC of human salivary gland. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-AMY1A / Salivary Amylase antibody IHC of human salivary gland. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(AMY1A monoclonal antibody, clone 2D4. Western blot of AMY1A expression in human pancreas.)

Western Blot (WB) (AMY1A monoclonal antibody, clone 2D4. Western blot of AMY1A expression in human pancreas.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
276
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,768 Da
NCBI Official Full Name
alpha-amylase 1
NCBI Official Synonym Full Names
amylase, alpha 1A (salivary)
NCBI Official Symbol
AMY1A
NCBI Official Synonym Symbols
AMY1
NCBI Protein Information
alpha-amylase 1; glycogenase; salivary alpha-amylase; salivary amylase alpha 1A; amylase, salivary, alpha-1A; 1,4-alpha-D-glucan glucanohydrolase 1
UniProt Protein Name
Alpha-amylase 1
UniProt Gene Name
AMY1A
UniProt Synonym Gene Names
AMY1
UniProt Entry Name
AMY1_HUMAN

NCBI Description

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

AMY1: Monomer. Belongs to the glycosyl hydrolase 13 family.

Protein type: Hydrolase; Secreted, signal peptide; Carbohydrate Metabolism - starch and sucrose; EC 3.2.1.1; Secreted

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space

Molecular Function: alpha-amylase activity; protein binding; metal ion binding

Biological Process: digestion; carbohydrate metabolic process

Research Articles on AMY1A

Similar Products

Product Notes

The AMY1A amy1a (Catalog #AAA246000) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 2D4] (IgG2a,k) to Human AMY1A / Salivary Amylase reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AMY1A / Salivary Amylase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000) Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Tissue lysate, Recombinant protein. Researchers should empirically determine the suitability of the AMY1A amy1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMY1A / Salivary Amylase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.