Rat anti-Mouse AMIGO3 Monoclonal Antibody | anti-Amigo3 antibody
AMIGO3 (Amphoterin-Induced Gene and ORF 3, Alivin-3)
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
PurifiedPurified by Protein G affinity chromatography.
Synonyms
AMIGO3; Monoclonal Antibody; AMIGO3 (Amphoterin-Induced Gene and ORF 3; Alivin-3); Anti -AMIGO3 (Amphoterin-Induced Gene and ORF 3; anti-Amigo3 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
8H161
Specificity
Recognizes mouse AMIGO3.
Purity/Purification
Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Applicable Applications for anti-Amigo3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry.
Dilution: Direct ELISA: 0.5- 1.0ug/ml detection limit for rmAMIGO3 is ~ 1ng/well.
Western Blot: 1-2ug/ml detection limit for rmAMIGO3 is ~ 25ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 25ug/ml.
Dilution: Direct ELISA: 0.5- 1.0ug/ml detection limit for rmAMIGO3 is ~ 1ng/well.
Western Blot: 1-2ug/ml detection limit for rmAMIGO3 is ~ 25ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 25ug/ml.
Immunogen
NS0-derived rmAMIGO3. (aa 20 - 378)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Amigo3 antibody
AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively.
Product Categories/Family for anti-Amigo3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,565 Da
NCBI Official Full Name
amphoterin-induced protein 3
NCBI Official Synonym Full Names
adhesion molecule with Ig like domain 3
NCBI Official Symbol
Amigo3
NCBI Protein Information
amphoterin-induced protein 3; AMIGO-3; alivin-3; transmembrane protein AMIGO3; amphoterin induced gene and ORF 3
UniProt Protein Name
Amphoterin-induced protein 3
Protein Family
UniProt Gene Name
Amigo3
UniProt Synonym Gene Names
Ali3
UniProt Entry Name
AMGO3_RAT
Uniprot Description
Function: May mediate heterophilic cell-cell interaction. May contribute to signal transduction through its intracellular domain.
Subunit structure: Binds AMIGO1 or AMIGO2.
Subcellular location: Membrane; Single-pass type I membrane protein
Potential.
Sequence similarities: Belongs to the immunoglobulin superfamily. AMIGO family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 6 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.Contains 1 LRRNT domain.
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Product Notes
The Amigo3 amigo3 (Catalog #AAA604054) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The AMIGO3 (Amphoterin-Induced Gene and ORF 3, Alivin-3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AMIGO3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Dilution: Direct ELISA: 0.5- 1.0ug/ml detection limit for rmAMIGO3 is ~ 1ng/well. Western Blot: 1-2ug/ml detection limit for rmAMIGO3 is ~ 25ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 25ug/ml. Researchers should empirically determine the suitability of the Amigo3 amigo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMIGO3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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