Mouse alpha-V/beta-3 Integrin Monoclonal Antibody

alpha-V/beta-3 Integrin, Human, mAb BV3

Cat. Num. AAA584338

• Reactivity: Reactivity: Human. Cross reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
• Synonyms: alpha-V/beta-3 Integrin; Monoclonal Antibody; Human; mAb BV3; Vitronectin receptor; integrin alpha-v/beta-3; cd51/cd61; anti-alpha-V/beta-3 Integrin antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-alpha-V/beta-3 Integrin antibody: Flow Cytometry (FC/FACS), Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), P
• Application Notes: Application Notes: FC: Antibody BV3 stains the extracellular domain of integrin alphavbeta3. The cells were fixed in 4% paraformaldehyde before before analysis. Negative control the primary antibody was omitted. (Ref.2); Application Use: For flow cytometry and immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Positive Control: HUVEC cells
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-alpha-V/beta-3 Integrin antibody

Product Description: The monoclonal antibody BV3 recognizes human alpha-V/beta-3 integrin present on human cells. Integrins are a superfamily of alphabeta heterodimeric cell-surface adhesion receptors found in many species. They are expressed on a variety of cells and mediate numerous physiological processes, including inflammation, migration, adhesion and proliferation. The beta3 family consist of 2 members: alphaIIbbeta3 and alphavbeta3, which mediate cell-cell and cell-ECM interactions and are important for cellular migration, regulation of gene expression, cell survival, adhesion and differentiation. All processes which are involved in tissue development, angiogenesis and thrombosis. Each subunit consist of an extracellular domain, a single transmembrane segment and a cytoplasmic tail. They connect to the actin cytoskeleton via adaptor proteins that bind theircytoplasmic tails. Cell matrix adhesions also act as signaling units by their capacity to organize the actin cytoskeleton and to accumulate various signaling intermediates. Integrin alphavbeta3 was originally identified as the vitronectin receptor. Nevertheless, other ligands include fibrinogen, fibronectin, laminin, thrombospondin, Von Willebrand factor, tenascin, osteopontin and several forms of collagen. The interactions of integrin alphavbeta3 to those ligands is mediated by the RGD (Arg-Gly-Asp) sequence motif present in these proteins. Deregulation of beta3 integrins is involved in e.g. autoimmune diseases, cardiovascular disorders, transplant rejection and tumorigenesis. In contribution to the latter, integrin alphavbeta3 contribute by supporting growth of small (tumor) blood vessels thereby potentiating the metastatic potential. Overexpression of integrin alphavbeta3 has been demonstrated in various tumors and activated endothelium.

References

1. Newton, S et al.; Electroconvulsive seizure increases adult hippocampal angiogenesis in rats. Eur J neurosc. 2006, 24:819-828
2. Merkel, O et al.; Integrin alphavbeta3 targeted gene delivery using RGD peptidomimetic conjugates with copolymers of PRGylated poly(ethyleneimine). Bioconj chem.. 2009, 20:1270-1280
3. Dare, E et al.; Fibrin sealants from fresh/frozen plasma as scaffolds for in vitro articular cartilage regeneration. Tissue engineering, 2009, 15:2285