Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using α-Smooth Muscle Actin (ACTA2) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Rabbit alpha-Smooth Muscle Actin (ACTA2) Monoclonal Antibody | anti-ACTA2 antibody
alpha-Smooth Muscle Actin (ACTA2) Rabbit mAb
IHC: 1:50-1:200
IF: 1:50-1:200
Customer Validation:
IHC: Mouse
WB: Fruit Fly, Zea mays, Mouse, Rat
IHC: Rat
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using α-Smooth Muscle Actin (ACTA2) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using α-Smooth Muscle Actin (ACTA2) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using α-Smooth Muscle Actin (ACTA2) Rabbit mAb at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using α-Smooth Muscle Actin (ACTA2) Rabbit mAb at dilution of 1:100 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of smooth muscle of human colon carcinoma using α-Smooth Muscle Actin (ACTA2) Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of smooth muscle of human colon carcinoma using α-Smooth Muscle Actin (ACTA2) Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of mouse colon using α-Smooth Muscle Actin (ACTA2) Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of mouse colon using α-Smooth Muscle Actin (ACTA2) Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of rat colon using α-Smooth Muscle Actin (ACTA2) Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of rat colon using α-Smooth Muscle Actin (ACTA2) Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Uniprot Description
ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.
Protein type: Contractile; Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 10q23.3
Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton
Molecular Function: protein kinase binding; ATP binding
Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction
Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6
Research Articles on ACTA2
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Product Notes
The ACTA2 acta2 (Catalog #AAA9143096) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha-Smooth Muscle Actin (ACTA2) Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's alpha-Smooth Muscle Actin (ACTA2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200 Customer Validation: IHC: Mouse WB: Fruit Fly, Zea mays, Mouse, Rat IHC: Rat. Researchers should empirically determine the suitability of the ACTA2 acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-Smooth Muscle Actin (ACTA2), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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