Western Blot (WB)
(Western Blot (WB) analysis of alpha-SMA Rabbit mAb at 1:1000 dilutionLane1:A549 whole cell lysateLane2:Hela whole cell lysateLane3:NIH/3T3 whole cell lysateLane4:The heart tissue lysate of Mouse)
Rabbit alpha-SMA Monoclonal Antibody | anti-alpha-SMA antibody
alpha-SMA Rabbit Monoclonal Antibody
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of alpha-SMA Rabbit mAb at 1:1000 dilutionLane1:A549 whole cell lysateLane2:Hela whole cell lysateLane3:NIH/3T3 whole cell lysateLane4:The heart tissue lysate of Mouse)
Western Blot (WB)
(Western Blot (WB) analysis of alpha-SMA Rabbit mAb at 1:1000 dilutionLane1:A549 whole cell lysateLane2:Hela whole cell lysateLane3:NIH/3T3 whole cell lysateLane4:The heart tissue lysate of Mouse)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-alpha-SMA antibody. Counter stained with hematoxylin.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with alpha-SMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with alpha-SMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]
Uniprot Description
ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.
Protein type: Contractile; Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 10q23.3
Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton
Molecular Function: protein kinase binding; ATP binding
Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction
Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6
Research Articles on alpha-SMA
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Product Notes
The alpha-SMA acta2 (Catalog #AAA3014126) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha-SMA Rabbit Monoclonal Antibody reacts with Human, Mouse, Rat, Zebra fish and may cross-react with other species as described in the data sheet. AAA Biotech's alpha-SMA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the alpha-SMA acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-SMA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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