Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5. Tissue: Burned Skin. Species: Human. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:200.)
Mouse Alginate Monoclonal Antibody
Alginate Antibody: ATTO 390
Reactivity
Species Independent
Applications
Immunohistochemistry, Western Blot
Synonyms
Alginate; Monoclonal Antibody; Alginate Antibody: ATTO 390; anti-Alginate antibody
Host
Mouse
Reactivity
Species Independent
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4B10-1C5
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-Alginate antibody
Immunohistochemistry (IHC), Western Blot (WB)
Conjugate
ATTO 390
Storage Buffer
PBS pH7.4, 50% glycerol
Preparation and Storage
-20 degree C
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5. Tissue: Burned Skin. Species: Human. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:200.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5. Tissue: Burned Skin. Species: Human. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:200.)
Western Blot (WB)
(Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5. Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa.)
Western Blot (WB)
(Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5. Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa.)
Related Product Information for anti-Alginate antibody
Background Info: Binds selectively to a BSA-conjugated alginate, but not to unconjugated BSA.
Scientific Background: Sodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+. Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds (1), as well as in many other cell types (2).
Scientific Background: Sodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+. Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds (1), as well as in many other cell types (2).
Product Categories/Family for anti-Alginate antibody
References
1. Zhai P., Chen X.B., and Schreyer D.J. (2013) Biofabrication. 5(1): 015009. 2. Selimoglu S.M., Ayyildiz-Tamis D., Gurhan I.D., and Elibol M. (2012) J Biosci Bioeng. 113(2):233-238.
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Product Notes
The Alginate (Catalog #AAA801033) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Alginate Antibody: ATTO 390 reacts with Species Independent and may cross-react with other species as described in the data sheet. AAA Biotech's Alginate can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the Alginate for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alginate, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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