Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Mouse anti-Human AKR1C2 Monoclonal Antibody | anti-AKR1C2 antibody
AKR1C2 (Aldo-keto Reductase Family 1 Member C2, 3-alpha-HSD3, Chlordecone Reductase Homolog HAKRD, Dihydrodiol Dehydrogenase 2, DD-2, DD2, Dihydrodiol Dehydrogenase/Bile Acid-binding Protein, DD/BABP, Trans-1,2-dihydrobenzene-1,2-diol Dehydrogenase, Type
Gene Names
AKR1C2; DD; DD2; TDD; BABP; DD-2; DDH2; HBAB; HAKRD; MCDR2; SRXY8; DD/BABP; AKR1C-pseudo
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AKR1C2; Monoclonal Antibody; AKR1C2 (Aldo-keto Reductase Family 1 Member C2; 3-alpha-HSD3; Chlordecone Reductase Homolog HAKRD; Dihydrodiol Dehydrogenase 2; DD-2; DD2; Dihydrodiol Dehydrogenase/Bile Acid-binding Protein; DD/BABP; Trans-1; 2-dihydrobenzene-1; 2-diol Dehydrogenase; Type; anti-AKR1C2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3C11
Specificity
Recognizes human AKR1C2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-AKR1C2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Sandwich ELISA: The detection limit is ~0.3ng/ml as a capture antibody.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa224-323 from human AKR1C2 (AAH63574) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
EEPWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEMKAIDGLNRNVRYLTLDIFAGPPNYPFSDEY
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Western Blot (WB)
(Western Blot analysis of AKR1C2 expression in transfected 293T cell line by AKR1C2 monoclonal antibody. Lane 1: AKR1C2 transfected lysate (36.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of AKR1C2 expression in transfected 293T cell line by AKR1C2 monoclonal antibody. Lane 1: AKR1C2 transfected lysate (36.7kD). Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged AKR1C2 is ~0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged AKR1C2 is ~0.3ng/ml as a capture antibody.)
Product Categories/Family for anti-AKR1C2 antibody
References
1. Aldo-keto reductases AKR1C1, AKR1C2 and AKR1C3 may enhance progesterone metabolism in ovarian endometriosis. Hevir N, Vouk K, Sinkovec J, Ribic-Pucelj M, Lanisnik Rizner T.Chem Biol Interact. 2011 Jan 11. 2.Aldo-keto reductase 1C2 fails to metabolize doxorubicin and daunorubicin in vitro. Takahashi RH, Bains OS, Pfeifer TA, Grigliatti TA, Reid RE, Riggs KW.Drug Metab Dispos. 2008 Jun;36(6):991-4. Epub 2008 Mar 5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
15,748 Da
NCBI Official Full Name
Homo sapiens aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III), mRNA
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member C2
NCBI Official Symbol
AKR1C2
NCBI Official Synonym Symbols
DD; DD2; TDD; BABP; DD-2; DDH2; HBAB; HAKRD; MCDR2; SRXY8; DD/BABP; AKR1C-pseudo
NCBI Protein Information
aldo-keto reductase family 1 member C2
Protein Family
NCBI Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Research Articles on AKR1C2
Similar Products
Product Notes
The AKR1C2 (Catalog #AAA6129941) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AKR1C2 (Aldo-keto Reductase Family 1 Member C2, 3-alpha-HSD3, Chlordecone Reductase Homolog HAKRD, Dihydrodiol Dehydrogenase 2, DD-2, DD2, Dihydrodiol Dehydrogenase/Bile Acid-binding Protein, DD/BABP, Trans-1,2-dihydrobenzene-1,2-diol Dehydrogenase, Type reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKR1C2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Sandwich ELISA: The detection limit is ~0.3ng/ml as a capture antibody. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AKR1C2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKR1C2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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