Immunofluorescence (IF)
(Immunofluorescence staining of Human AK4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human AK4 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Mouse anti-Human AK3L1 Monoclonal Antibody | anti-AK4 antibody
Anti-AK3L1 Antibody, Mouse Monoclonal
No cross-reactivity in ELISA with Human CACM5, Human PTPRA
FC/FACS/FCM: 1:25-1:100
Immunofluorescence (IF)
(Immunofluorescence staining of Human AK4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human AK4 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human AK4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human AK4 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human AK4 expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human AK4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human AK4 expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human AK4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]
Uniprot Description
AK3L1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. May also be active with GTP. Belongs to the adenylate kinase family.
Protein type: Nucleotide Metabolism - purine; EC 2.7.4.10; EC 2.7.4.6; Mitochondrial; Kinase, other
Chromosomal Location of Human Ortholog: 1p31.3
Cellular Component: mitochondrion; mitochondrial matrix
Molecular Function: GTP binding; nucleoside diphosphate kinase activity; adenylate kinase activity; nucleoside triphosphate adenylate kinase activity; ATP binding
Biological Process: response to drug; ATP metabolic process; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; ADP biosynthetic process; brain development; liver development; AMP metabolic process; GTP metabolic process
Research Articles on AK4
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Product Notes
The AK4 ak4 (Catalog #AAA8106393) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-AK3L1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AK3L1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the AK4 ak4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AK3L1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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