Western Blot (WB)
Mouse anti-Human Ajuba Monoclonal Antibody | anti-AJUBA antibody
Mouse Anti-Ajuba [21G4]
Western Blot (WB)
Western Blot (WB)
NCBI and Uniprot Product Information
Uniprot Description
JUB: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell- cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1- induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Interacts with GRB2, PIP5K1B and SLC1A2. Interacts with AURKA; the interaction occurs during mitosis and both proteins are phosphorylated as they form a complex. Interacts with CTNNA1 and with F-actin. Interacts with LATS2; the interaction occurs during mitosis and the complex regulates organization of the spindle apparatus through recruitment of TUBG to the centrosome. Forms a complex with SQSTM1, PRKCZ and TRAF6. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly (via the LIM domains) with GFI1; the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes, and is independent of the GFI1 SNAG domain. Interacts with HDAC1, HDAC2 and HDAC3. Interacts with SNAI2/SLUG (via SNAG domain) and SCRT1 (via SNAG domain). Interacts with EIF4E, EIF2C1, EIF2C2, DCP2, DDX6, LATS1, LATS2, SAV1, EGLN2/PHD1 and EGLN3/PHD3. Interacts (via LIM domains) with isoform 1 and isoform 3 of VHL. Interacts (via LIM domains) with SNAI1 (via SNAG domain). Belongs to the zyxin/ajuba family.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: transcription factor complex; focal adhesion; lamellipodium; plasma membrane; microtubule organizing center; intercellular junction; cytosol; nucleus
Molecular Function: actin filament binding; protein binding; zinc ion binding; chromatin binding; transcription corepressor activity; alpha-catenin binding
Biological Process: focal adhesion formation; lamellipodium biogenesis; negative regulation of kinase activity; regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; glycerophospholipid biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cellular biosynthetic process; miRNA-mediated gene silencing; regulation of cell migration; positive regulation of MAP kinase activity; positive regulation of protein complex assembly; calcium-dependent cell-cell adhesion; response to hypoxia; mitotic cell cycle; G2/M transition of mitotic cell cycle; wound healing, spreading of epidermal cells
Research Articles on AJUBA
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Product Notes
The AJUBA ajuba (Catalog #AAA375105) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Ajuba [21G4] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ajuba can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Optimal antibody dilution should be determined by titration, however as a guideline try at; WB: 1/100. Predicted molecular weight: 57 kDa. Researchers should empirically determine the suitability of the AJUBA ajuba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ajuba, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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