Mouse Aggrecan, C-terminal neoepitope NITEGE Monoclonal Antibody
Monocloncal Antibody NITEGE (Clone BC13)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Affinity purified on protein G
Synonyms
Aggrecan; C-terminal neoepitope NITEGE; Monoclonal Antibody; Monocloncal Antibody NITEGE (Clone BC13); Aggrecan Antibody; anti-Aggrecan; C-terminal neoepitope NITEGE antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
BC-13
Specificity
Recognizes the aggrecanase (ADAMTS-1, -4 & -5)-generated C-terminal neoepitope ...TEGE after cleavage between amino acids ..EGE373 and 374ARG.. within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration).
Purity/Purification
Affinity purified on protein G
Form/Format
Liquid, PBS, no preservatives.
Applicable Applications for anti-Aggrecan, C-terminal neoepitope NITEGE antibody
Western Blotting (WB), ELISA (EIA), Immunohistochemistry (IHC).
Application Notes
WB: Suggested dilution: 1:100. Detects a a band of approximately 60-70kDa.
Myeloma
x63-Ag8.653
Light Chain Type
Kappa
Immunogen
NITEGE synthetic peptide conjugate
Technical Notes
Samples are usually deglycosylated using 0.01 Units chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
Cross Reactivity
Human, Cow, Dog, Pig, Horse
Preparation and Storage
The antibody is stable until the expiry date given on the label if stored at -20°C. Repeated freezing and thawing should be avoided. Aliquoting is recommended.
Related Product Information for anti-Aggrecan, C-terminal neoepitope NITEGE antibody
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue.
This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue.
Product Categories/Family for anti-Aggrecan, C-terminal neoepitope NITEGE antibody
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Product Notes
The Aggrecan, C-terminal neoepitope NITEGE (Catalog #AAA442004) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Aggrecan, C-terminal neoepitope NITEGE can be used in a range of immunoassay formats including, but not limited to, Western Blotting (WB), ELISA (EIA), Immunohistochemistry (IHC). WB: Suggested dilution: 1:100. Detects an a band of approximately 60-70kDa. Researchers should empirically determine the suitability of the Aggrecan, C-terminal neoepitope NITEGE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aggrecan, C-terminal neoepitope NITEGE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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