Rabbit anti-Human AFP  Monoclonal Antibody | anti-AFP  antibody

Rabbit anti Human AFP  Monoclonal Antibody

Cat. Num. AAA2544347

• Gene Names: AFP; AFPD; FETA; HPAFP
• Reactivity: Human
• Applications: ELISA
• Synonyms: AFP ; Monoclonal Antibody; Rabbit anti Human AFP Monoclonal Antibody; anti-AFP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 609

• Applicable Applications for anti-AFP antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human AFP/alpha-fetoprotein. The detection limit for Human AFP/alpha-fetoprotein is approximately 0.00245 ng/well.
• Immunogen: Recombinant Human AFP/alpha-fetoprotein protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-AFP  antibody

Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver (1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).

NCBI and Uniprot Product Information

• NCBI GI #: 4501989
• NCBI GeneID: 174
• NCBI GenBank Nucleotide #: NM_001134.2
• Molecular Weight: 68,678 Da
• NCBI Official Full Name: alpha-fetoprotein
• NCBI Official Synonym Full Names: alpha fetoprotein
• NCBI Official Symbol: AFP
• NCBI Official Synonym Symbols: AFPD; FETA; HPAFP
• NCBI Protein Information: alpha-fetoprotein
• UniProt Protein Name: Alpha-fetoprotein
• UniProt Gene Name: AFP
• UniProt Synonym Gene Names: HPAFP
• UniProt Entry Name: FETA_HUMAN

NCBI Description

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Uniprot Description

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cytoplasm

Molecular Function: protein binding

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of