Western Blot (WB)
(Figure 1. Western blot analysis of AFF4 using anti-AFF4 antibody (MBS1754030).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human CACO-2 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human MDA-MB-453 whole cell lysatesLane 6: human PANC-1 whole cell lysatesLane 7: human SW620 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- AFF4 antigen affinity purified monoclonal antibody (Catalog # MBS1754030) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for AFF4 at approximately 150KD. The expected band size for AFF4 is at 150KD. )
Mouse anti-Human AFF4 Monoclonal Antibody | anti-AFF4 antibody
Anti-AFF4 Antibody (monoclonal, 8G12)
; anti-AFF4 antibody
IHC-P: 2-5ug/mlHuman|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of AFF4 using anti-AFF4 antibody (MBS1754030).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human CACO-2 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human MDA-MB-453 whole cell lysatesLane 6: human PANC-1 whole cell lysatesLane 7: human SW620 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- AFF4 antigen affinity purified monoclonal antibody (Catalog # MBS1754030) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for AFF4 at approximately 150KD. The expected band size for AFF4 is at 150KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of AFF4 using anti-AFF4 antibody (MBS1754030).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human CACO-2 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human MDA-MB-453 whole cell lysatesLane 6: human PANC-1 whole cell lysatesLane 7: human SW620 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- AFF4 antigen affinity purified monoclonal antibody (Catalog # MBS1754030) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for AFF4 at approximately 150KD. The expected band size for AFF4 is at 150KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-AFF4 antibody (MBS1754030).Overlay histogram showing 293T cells stained with MBS1754030 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- AFF4 Antibody (MBS1754030, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-AFF4 antibody (MBS1754030).Overlay histogram showing 293T cells stained with MBS1754030 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- AFF4 Antibody (MBS1754030, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Taki, T., Kano, H., Taniwaki, M., Sako, M., Yanagisawa, M., Hayashi, Y. AF5q31, a newly identified AF4-related gene, is fused to MLL in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). Proc. Nat. Acad. Sci. 96: 14535-14540, 1999.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011]
Uniprot Description
MCEF: a putative transcription factor. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). Genetic fusion of its gene with MLL has been observed in a subset of acute lymphoblastic leukemia patients. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleoplasm; transcription elongation factor complex; mitochondrion; nucleolus; nucleus
Molecular Function: protein binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; spermatid development
Disease: Chops Syndrome
Research Articles on AFF4
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Product Notes
The AFF4 aff4 (Catalog #AAA1754030) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-AFF4 Antibody (monoclonal, 8G12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AFF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM). WB: 0.25-0.5ug/ml|Human| IHC-P: 2-5ug/mlHuman| FC/FACS/FCM: 1-3ug/1x106 cells|Human|. Researchers should empirically determine the suitability of the AFF4 aff4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFF4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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