Testing Data
(IP western blot analysis was performed on human leukemia cell lines that harbor MLL chromosomal translocations and express MLL chimeric oncoproteins (indicated at tops). IP was performed with the antibodies indicated on the top of the gels, and the precipitates were immunoblotted with the antibodies indicated on the right. Antibody MBS190886 is shown as AF4. Anti-GST antibodies served as negative controls. Positive control: K562 cell lysates.Optimal dilutions/concentrations should be determined by the end user.)
Mouse AF4 Monoclonal Antibody | anti-AF4 antibody
Anti-AF4 Monoclonal Antibody
Testing Data
(IP western blot analysis was performed on human leukemia cell lines that harbor MLL chromosomal translocations and express MLL chimeric oncoproteins (indicated at tops). IP was performed with the antibodies indicated on the top of the gels, and the precipitates were immunoblotted with the antibodies indicated on the right. Antibody MBS190886 is shown as AF4. Anti-GST antibodies served as negative controls. Positive control: K562 cell lysates.Optimal dilutions/concentrations should be determined by the end user.)
Testing Data
(IP western blot analysis was performed on human leukemia cell lines that harbor MLL chromosomal translocations and express MLL chimeric oncoproteins (indicated at tops). IP was performed with the antibodies indicated on the top of the gels, and the precipitates were immunoblotted with the antibodies indicated on the right. Antibody MBS190886 is shown as AF4. Anti-GST antibodies served as negative controls. Positive control: K562 cell lysates.Optimal dilutions/concentrations should be determined by the end user.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
AF-4: A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein. Belongs to the AF4 family.
Protein type: Transcription factor; Oncoprotein
Chromosomal Location of Human Ortholog: 4q21
Cellular Component: transcription elongation factor complex
Molecular Function: protein binding; transcription factor activity
Biological Process: regulation of transcription, DNA-dependent
Research Articles on AF4
Similar Products
Product Notes
The AF4 aff1 (Catalog #AAA190886) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AF4 can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB). Immunoblotting: Use at 1-5ug/ml. A band of ~131kDa is detected. Researchers should empirically determine the suitability of the AF4 aff1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AF4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
